Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F11%3A00369489" target="_blank" >RIV/67985823:_____/11:00369489 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21460/11:00185826
Result on the web
<a href="http://dx.doi.org/10.1002/jemt.20965" target="_blank" >http://dx.doi.org/10.1002/jemt.20965</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jemt.20965" target="_blank" >10.1002/jemt.20965</a>
Alternative languages
Result language
angličtina
Original language name
Compensation of inhomogeneous fluorescence signal distribution in 2D images acquired by confocal microscopy
Original language description
In images acquired by confocal laser scanning microscopy (CLSM), regions corresponding to the same concentration of fluorophores in the specimen should be mapped to the same grayscale levels. However, in practice, due to multiple distortion effects, CLSMimages of even homogeneous specimen regions suffer from irregular brightness variations, e.g., darkening of image edges and lightening of the center. We present a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
JC - Computer hardware and software
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microscopy Research Technique
ISSN
1059-910X
e-ISSN
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Volume of the periodical
74
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
831-838
UT code for WoS article
000294401200007
EID of the result in the Scopus database
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