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Genetically modified S. cerevisiae strain lacking plasma membrane uptake systems for potassium cations

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F09%3A00337841" target="_blank" >RIV/67985823:_____/09:00337841 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Genetically modified S. cerevisiae strain lacking plasma membrane uptake systems for potassium cations

  • Original language description

    S.cerevisiae BYT1, BYT2 and BYT12 mutant strains lacking the TRK1 and/or TRK2 genes (encoding potassium uptake transporters) have been constructed. The TRK1 gene has been substituted by the LEU2 gene and the TRK2 has been substituted by the kanMX cassette. Finally, LEU2 and kanMX cassettes were replaced by cre-loxP system leaving a single loxP site. Unique GMO strains in the BY4741 background have been created to study heterologously expressed potassium transporters from both lower and higher eukaryotes

  • Czech name

  • Czech description

Classification

  • Type

    G<sub>funk</sub> - Functional sample

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2009

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Internal product ID

    S. cerevisiae BYT1, BYT2 a BYT12

  • Numerical identification

  • Technical parameters

    Mutantní kmeny S. cerevisiae BYT1, BYT2 a BYT12 s neschopností růstu na médiích s limitně nízkým obsahem draselných solí a hyperpolarizovanou plasmatickou membránou jsou vhodné pro heterologní expresi transportérů draslíku z jiných eukaryot

  • Economical parameters

    náklady v 300 000,- Kč, licenční poplatek 50 000,- Kč

  • Application category by cost

  • Owner IČO

    67985823

  • Owner name

    Fyziologický ústav AVČR, v.v

  • Owner country

    CZ - CZECH REPUBLIC

  • Usage type

    P - Využití výsledku jiným subjektem je v některých případech možné bez nabytí licence

  • Licence fee requirement

    N - Poskytovatel licence na výsledek nepožaduje licenční poplatek

  • Web page