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Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium and sodium cations

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F09%3A00337845" target="_blank" >RIV/67985823:_____/09:00337845 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Genetically modified S. cerevisiae strain lacking both importers and exporters of potassium and sodium cations

  • Original language description

    S. cerevisiae mutant strains lacking the genes encoding alkali-metal-cation exporters (NHA1, ENA1, ENA2, ENA5, TOK1) and potassium importers (TRK1, TRK2) have been constructed. The TRK1 gene has been substituted by the LEU2 gene; the three ENA genes, TOK1 and TRK2 genes have been replaced by the kanMX cassette. Finally, the LEU2 and kanMX cassettes from all strains were replaced by cre-loxP system leaving a single loxP site. Unique GMO strains in the BY4741 background lacking either 6 or 7 transportershave been created to study heterologously expressed sodium and potassium transporters from both lower and higher eukaryots

  • Czech name

  • Czech description

Classification

  • Type

    G<sub>funk</sub> - Functional sample

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2009

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Internal product ID

    S. cerevisiae BYT1245 a BYT12345

  • Numerical identification

  • Technical parameters

    Mutantní kmeny S. cerevisiae BYT1245 (nha1 ena1-5 trk1 trk2) a BYT12345 (nha1 ena1-5 tok1 trk1 trk2) s vysokou citlivostí k solím alkalických kationů a zároveň s neschopností růst na médiích s limitně nízkým obsahem draselných solí

  • Economical parameters

    náklady v 300 000,- Kč, licenční poplatek 50 000,- Kč

  • Application category by cost

  • Owner IČO

    67985823

  • Owner name

    Fyziologický ústav AVČR, v.v

  • Owner country

    CZ - CZECH REPUBLIC

  • Usage type

    P - Využití výsledku jiným subjektem je v některých případech možné bez nabytí licence

  • Licence fee requirement

    N - Poskytovatel licence na výsledek nepožaduje licenční poplatek

  • Web page