Dependence of spontaneous electrical activity and basal prolactin release on nonselective cation channels in pituitary lactotrophs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F12%3A00385667" target="_blank" >RIV/67985823:_____/12:00385667 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Dependence of spontaneous electrical activity and basal prolactin release on nonselective cation channels in pituitary lactotrophs

Original language description

In cultured anterior pituitary cells, including lactotrophs and immortalized GH3 cells, replacement of extracellular Na+ with large organic cations led to an instantaneous hyperpolarization of cell membranes that was associated with a cessation of spontaneous firing. Selective blockers of TRPC channels, inhibited electrical activity, Ca2+ influx and prolactin release in a concentration-dependent manner. Quantitative RT-PCR analysis revealed the high expression of mRNA transcripts for TRPC1 in both lactotrophs and GH3 cells. These results indicate that nonselective cation channels, presumably belonging to the TRPC family, contribute to the background depolarizing conductance and firing of action potentials with consequent contribution to Ca2+ influx andhormone release in lactotrophs and GH3 cells

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological Research

ISSN

0862-8408

e-ISSN

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Volume of the periodical

61

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

267-275

UT code for WoS article

000306508000005

EID of the result in the Scopus database

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