High Molecular Weight Forms of Mammalian Respiratory Chain Complex II
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F13%3A00396457" target="_blank" >RIV/67985823:_____/13:00396457 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/13:00396457
Result on the web
<a href="http://dx.doi.org/10.1371/journal.pone.0071869" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0071869</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0071869" target="_blank" >10.1371/journal.pone.0071869</a>
Alternative languages
Result language
angličtina
Original language name
High Molecular Weight Forms of Mammalian Respiratory Chain Complex II
Original language description
In this study, we focused on higher molecular weight forms of CII in mammalian cells and tissues, which can be detectable after solubilisation with mild detergent digitonin and clear native electrophoresis, and on to specific interactions of CII with complexes of oxidative phosphorylation pathway or TCA cycle. Native electrophoresis in combination with immunodetection revealed existence of CIIhmw structures, which are enzymatically active and differ in electrophoretic mobility between tissues and cultured cells. These CIIhmw are unstable and readily dissociate on to CII monomer and individual subunits. Isolated defects of OXPHOS complexes have no effect on CIIhmw formation, but they are destabilised in mtDNA-depleted, rho0 cells. Immunoprecipitation and electrophoretic experiments indicate that one of the binding partners of CII could be CV, interactions with other OXPHOS or TCA cycle complexes were not detected
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS ONE
ISSN
1932-6203
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
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UT code for WoS article
000323115800089
EID of the result in the Scopus database
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