Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F14%3A00427739" target="_blank" >RIV/67985823:_____/14:00427739 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1038/nprot.2014.009" target="_blank" >http://dx.doi.org/10.1038/nprot.2014.009</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/nprot.2014.009" target="_blank" >10.1038/nprot.2014.009</a>

Result language
angličtina
Original language name
Germline transgenesis in rabbits by pronuclear microinjection of Sleeping Beauty transposons
Original language description
We describe a protocol for high-efficiency germline transgenesis and sustained transgene expression in rabbits by using the Sleeping Beauty (SB) transposon system. The protocol is based on co-injection into the pronuclei of fertilized oocytes of synthetic mRNA encoding the SB100X hyperactive transposase together with plasmid DNAcarrying a transgene construct flanked by binding sites for the transposase
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/LH12061" target="_blank" >LH12061: Identification of molecular and functional basis of pathophysiological phenotypes in the spontaneously hypertensive rat</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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