Germline transgenesis in rodents by pronuclear microinjection of Sleeping Beauty transposons

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F14%3A00428045" target="_blank" >RIV/67985823:_____/14:00428045 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/nprot.2014.008" target="_blank" >http://dx.doi.org/10.1038/nprot.2014.008</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/nprot.2014.008" target="_blank" >10.1038/nprot.2014.008</a>

Result language

angličtina

Original language name

Germline transgenesis in rodents by pronuclear microinjection of Sleeping Beauty transposons

Original language description

We described a protocol for high-efficiency germline transgenesis and sustained transgene expression in two important biomedical models, the mouse and the rat, by using the Sleeping Beauty transposon system. The procedure is based on co-injection of synthetic mRNA encoding the SB100X hyperactive transposase, together with circular plasmid DNAcarrying a transgene construct flanked by binding sites for the transposase, into the pronuclei of fertilized oocytes

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature Protocols

ISSN

1754-2189

e-ISSN

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Volume of the periodical

9

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

21

Pages from-to

773-793

UT code for WoS article

000333752100004

EID of the result in the Scopus database

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