Growth of human adipose-derived stem cells on Ti-6Al-4V alloy with various surface modifications

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F15%3A00470319" target="_blank" >RIV/67985823:_____/15:00470319 - isvavai.cz</a>

Alternative codes found

RIV/00064211:_____/15:N0000002

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Growth of human adipose-derived stem cells on Ti-6Al-4V alloy with various surface modifications

Original language description

Adipose-derived stem cells (ASCs) are of a great importance for bone tissue engineering. We compared ASCs obtained by liposuction under two pressures (-200 mmHg and -700 mmHg). The number and proliferation activity of cells isolated under -700 mmHg (ASC-H) were higher than in cells isolated under -200 mmHg (ASC-L). However, the ASC-L was more active in osteogenic differentiation, as manifested by a higher intensity of fluorescence of alkaline phosphatase and osteocalcin in these cells. The adhesion and growth of ASCs were then studied on Ti-6Al-4V samples either unmodified (K, roughness parameter R-a = 280 nm) or modified by shot blasting and tarnishing A, R-a = 200 nm), vibratory finishing (B, R-a = 100 nm) and vibratory finishing, shot blasting and polishing (C, R-a = 80 nm). All modified samples were more wettable than the K samples. On day 1 after seeding, the size of cell spreading area on some modified samples was lower than on K samples, namely on B samples in ASC-L, on B and C in ASC-H and on A in control MG-63 cells. On day 3, the cell number on some modified samples became higher than on K samples, namely on C samples (ASC-L), on B (ASC-H) and on B and C s (MG-63 cells). On day 7, the numbers of ASC-H and MG-63 cells on all modified samples evened out and became significantly higher than on K samples. On ASC-L samples, the highest cell numbers were obtained on A samples. Thus, all studied modifications of Ti-6Al-4V enhanced growth of ASCs and human osteoblast-like MG-63 cells.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NANOCON 2015: 7th International Conference on Nanomaterials - Research and Application, Conference Proceedings

ISBN

978-80-87294-63-5

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

466-471

Publisher name

TANGER, spol. s r.o.

Place of publication

Ostrava

Event location

Brno

Event date

Oct 14, 2015

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000374708800082