In vitro and in vivo activation of mitochondrial membrane permeability transition pore using triiodothyronine

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00461898" target="_blank" >RIV/67985823:_____/16:00461898 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/16:10323879

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

In vitro and in vivo activation of mitochondrial membrane permeability transition pore using triiodothyronine

Original language description

Using a novel method for evaluating mitochondrial swelling we studied the effect of calcium (Ca2+), phosphate (Pi), and triiodothyronine (T3) on the opening of mitochondrial membrane permeability transition pore and how they interact in the activation of swelling process. We found that 0.1 mM Pi, 50 µM Ca2+ and 25 µM T3 when added separately increase the swelling rate to about 10 % of maximal values when all three factors are applied simultaneously. Our findings document that under experimental conditions in which Ca2+ and Pi are used as activating factors, the addition of T3 doubled the rate of swelling. T3 has also an activating effect on mitochondrial membrane potential. The T3 activating effect was also found after in vivo application of T3. Our data thus demonstrate that T3 has an important role in opening the mitochondrial membrane permeability pore and activates the function of the two key physiological swelling inducers, calcium and phosphate ions.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GB14-36804G" target="_blank" >GB14-36804G: Centre of mitochondrial biology and pathology (MITOCENTRE)</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological Research

ISSN

0862-8408

e-ISSN

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Volume of the periodical

65

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

321-331

UT code for WoS article

000382115000014

EID of the result in the Scopus database

2-s2.0-84975523404