Modification of Calcium Retention Capacity of Rat Liver Mitochondria by Phosphate and Tert-Butyl Hydroperoxide
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00504504" target="_blank" >RIV/67985823:_____/19:00504504 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/19:10393759
Result on the web
<a href="https://www.biomed.cas.cz/physiolres/pdf/2019/68_59.pdf" target="_blank" >https://www.biomed.cas.cz/physiolres/pdf/2019/68_59.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.33549/physiolres.933912" target="_blank" >10.33549/physiolres.933912</a>
Alternative languages
Result language
angličtina
Original language name
Modification of Calcium Retention Capacity of Rat Liver Mitochondria by Phosphate and Tert-Butyl Hydroperoxide
Original language description
By determining the calcium retention capacity (CRC) of rat liver mitochondria, we confirmed and extended previous observations describing the activation of mitochondrial swelling by phosphate and tert-butyl hydroperoxide (t-BHP). Using CRC measurements, we showed that both phosphate and t-BHP decrease the extent of calcium accumulation required for the full mitochondrial permeability transition pore (MPTP) opening to 35 % of control values and to only 15 % when both phosphate and t-BHP are present in the medium. When changes in fluorescence were evaluated at higher resolution, we observed that in the presence of cyclosporine A fluorescence values return after each Ca2+ addition to basal values obtained before the Ca2+ addition. This indicates that the MPTP remains closed. However, in the absence of cyclosporine A, the basal fluorescence after each Ca2+ addition continuously increased. This increase was potentiated both by phosphate and t-BHP until the moment when the concentration of intramitochondrial calcium required for the full opening of the MPTP was reached. We conclude that in the absence of cyclosporine A, the MPTP is slowly opened after each Ca2+ addition and that this rate of opening can be modified by various factors such as the composition of the media and the experimental protocol used.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30105 - Physiology (including cytology)
Result continuities
Project
<a href="/en/project/LTC17044" target="_blank" >LTC17044: Liver mitochondria - focus on Age, Lifestyle, Environment</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiological Research
ISSN
0862-8408
e-ISSN
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Volume of the periodical
68
Issue of the periodical within the volume
1
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
7
Pages from-to
59-65
UT code for WoS article
000460617400006
EID of the result in the Scopus database
2-s2.0-85062586692