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Modification of Calcium Retention Capacity of Rat Liver Mitochondria by Phosphate and Tert-Butyl Hydroperoxide

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00504504" target="_blank" >RIV/67985823:_____/19:00504504 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11150/19:10393759

  • Result on the web

    <a href="https://www.biomed.cas.cz/physiolres/pdf/2019/68_59.pdf" target="_blank" >https://www.biomed.cas.cz/physiolres/pdf/2019/68_59.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.33549/physiolres.933912" target="_blank" >10.33549/physiolres.933912</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Modification of Calcium Retention Capacity of Rat Liver Mitochondria by Phosphate and Tert-Butyl Hydroperoxide

  • Original language description

    By determining the calcium retention capacity (CRC) of rat liver mitochondria, we confirmed and extended previous observations describing the activation of mitochondrial swelling by phosphate and tert-butyl hydroperoxide (t-BHP). Using CRC measurements, we showed that both phosphate and t-BHP decrease the extent of calcium accumulation required for the full mitochondrial permeability transition pore (MPTP) opening to 35 % of control values and to only 15 % when both phosphate and t-BHP are present in the medium. When changes in fluorescence were evaluated at higher resolution, we observed that in the presence of cyclosporine A fluorescence values return after each Ca2+ addition to basal values obtained before the Ca2+ addition. This indicates that the MPTP remains closed. However, in the absence of cyclosporine A, the basal fluorescence after each Ca2+ addition continuously increased. This increase was potentiated both by phosphate and t-BHP until the moment when the concentration of intramitochondrial calcium required for the full opening of the MPTP was reached. We conclude that in the absence of cyclosporine A, the MPTP is slowly opened after each Ca2+ addition and that this rate of opening can be modified by various factors such as the composition of the media and the experimental protocol used.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30105 - Physiology (including cytology)

Result continuities

  • Project

    <a href="/en/project/LTC17044" target="_blank" >LTC17044: Liver mitochondria - focus on Age, Lifestyle, Environment</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Physiological Research

  • ISSN

    0862-8408

  • e-ISSN

  • Volume of the periodical

    68

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    7

  • Pages from-to

    59-65

  • UT code for WoS article

    000460617400006

  • EID of the result in the Scopus database

    2-s2.0-85062586692