Proteomic analysis of human tooth pulp proteomes – Comparison of caries-resistant and caries-susceptible

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00462855" target="_blank" >RIV/67985823:_____/16:00462855 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.jprot.2016.04.022" target="_blank" >http://dx.doi.org/10.1016/j.jprot.2016.04.022</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jprot.2016.04.022" target="_blank" >10.1016/j.jprot.2016.04.022</a>

Result language

angličtina

Original language name

Proteomic analysis of human tooth pulp proteomes – Comparison of caries-resistant and caries-susceptible

Original language description

Most people in the world suffer from dental caries, > 90% of adults experience caries on enamel and root surfaces during their life. However, the overall roles of all factors in the development of dental caries still remain unclear and are worthy of recent investigation. In this study we used a proteomic 2D-DIGE approach in connection with MS/MS to investigate the different protein abundances in the tooth pulp of human third molars obtained from caries-resistant and caries-susceptible people. Statistical analysis of the two protein maps obtained on large gel (17 cm length) and mini gel (7 cm length) followed by nLC-MS/MS analysis enabled the identification of 16 significantly changed spots with unique protein identifications corresponding to 12 non-redundant proteins. Seven proteins exhibited higher and four proteins exhibited lower expression in the caries-resistant samples compared to the caries-susceptible samples. Additionally, one protein (alpha-1-antitrypsin) exhibited both expressions (up and down). Most of the differentially expressed proteins were associated with protein metabolism, energy production, cytoskeletal organization and transport. These differentially expressed proteins are likely involved in the natural resistance or susceptibility of humans to the development of dental caries and suggest that the resistance mechanism is multifactorial.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FF - ENT (ie. ear, nose, throat), ophthalmology, dentistry

OECD FORD branch

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Project

<a href="/en/project/NT14324" target="_blank" >NT14324: Comparative study of protein content of saliva, dental pellicle and teeth in relation to dental decay</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Proteomics

ISSN

1874-3919

e-ISSN

—

Volume of the periodical

145

Issue of the periodical within the volume

Aug 11

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

127-136

UT code for WoS article

000383931000013

EID of the result in the Scopus database

2-s2.0-84964864688