Lack of cortical endoplasmic reticulum protein Ist2 alters sodium accumulation in Saccharomyces cerevisiae cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F17%3A00474850" target="_blank" >RIV/67985823:_____/17:00474850 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/femsyr/fox011" target="_blank" >http://dx.doi.org/10.1093/femsyr/fox011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/femsyr/fox011" target="_blank" >10.1093/femsyr/fox011</a>

Result language

angličtina

Original language name

Lack of cortical endoplasmic reticulum protein Ist2 alters sodium accumulation in Saccharomyces cerevisiae cells

Original language description

The maintenance of intracellular alkali-metal-cation homeostasis is a fundamental property of all living organisms, including the yeast Saccharomyces cerevisiae. Several transport systems are indispensable to ensure proper alkali-metal-cation levels in the yeast cytoplasm and organelles. Ist2 is an endoplasmic reticulum (ER)-resident protein involved, together with other tethering proteins, in the formation of contacts between the plasma and ER membranes. As IST2 gene deletion was shown to influence yeast growth in the presence of sodium, we focused on the roles of Ist2 in the cell response to the presence of various concentrations of alkali metal cations, and its interactions with characterised plasma membrane alkali-metal-cation transporters. Most importantly, we show that, in BY4741 background, the lack of Ist2 results in the accumulation of higher amounts of sodium when the cells are exposed to the presence of this cation, demonstrating the importance of Ist2 for the maintenance of low intracellular levels of toxic sodium. As the function and localisation of alkali-metal-cation exporters is not affected in ist2 Delta cells, IST2 deletion results in an increased non-specific uptake of sodium to cells. Moreover, the deletion of IST2 influences relative cell membrane potential, pH(in) and the growth of cells in the presence of a limiting K+ concentration.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10612 - Mycology

Project

<a href="/en/project/LH14297" target="_blank" >LH14297: Regulation of intracellular pH, calcium and potassium homeostases in budding yeast</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FEMS Yeast Research

ISSN

1567-1356

e-ISSN

—

Volume of the periodical

17

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

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UT code for WoS article

000400577600006

EID of the result in the Scopus database

2-s2.0-85019686849