Antioxidant tempol suppresses heart cytosolic phospholipase A(2)alpha stimulated by chronic intermittent hypoxia
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F17%3A00477749" target="_blank" >RIV/67985823:_____/17:00477749 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/17:10368553 RIV/00216208:11110/17:10368553
Result on the web
<a href="http://dx.doi.org/10.1139/cjpp-2017-0022" target="_blank" >http://dx.doi.org/10.1139/cjpp-2017-0022</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1139/cjpp-2017-0022" target="_blank" >10.1139/cjpp-2017-0022</a>
Alternative languages
Result language
angličtina
Original language name
Antioxidant tempol suppresses heart cytosolic phospholipase A(2)alpha stimulated by chronic intermittent hypoxia
Original language description
Adaptation to chronic intermittent hypoxia (CIH) is associated with reactive oxygen species (ROS) generation implicated in the improved cardiac tolerance against acute ischemia-reperfusion injury. Phospholipases A2 (PLA2s) play an important role in cardiomyocyte phospholipid metabolism influencing membrane homeostasis. Here we aimed to determine the effect of CIH (7000 m, 8 h/day, 5 weeks) on the expression of cytosolic PLA(2) (cPLA(2)alpha), its phosphorylated form (p-cPLA(2)alpha), calcium-independent (iPLA2), and secretory (sPLA2IIA) at protein and mRNA levels, as well as fatty acids (FA) profile in left ventricular myocardium of adult male Wistar rats. Chronic administration of antioxidant tempol was used to verify the ROS involvement in CIH effect on PLA2s expression and phospholipid FA remodeling. While CIH did not affect PLA2s mRNA levels, it increased the total cPLA(2)alpha protein in cytosol and membranes (by 191% and 38%, respectively) and p-cPLA(2)alpha (by 23%) in membranes. Onthe contrary, both iPLA2 and sPLA2IIA were downregulated by CIH. CIH further decreased phospholipid n-6 polyunsaturated FA (PUFA) and increased n-3 PUFA proportion. Tempol treatment prevented only CIH-induced cPLA(2)alpha up-regulation and its phosphorylation on Ser505. Our results show that CIH diversely affect myocardial PLA2s and suggest that ROS are responsible for the activation of cPLA(2)alpha under these conditions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Canadian Journal of Physiology and Pharmacology
ISSN
0008-4212
e-ISSN
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Volume of the periodical
95
Issue of the periodical within the volume
8
Country of publishing house
CA - CANADA
Number of pages
8
Pages from-to
920-927
UT code for WoS article
000407430100007
EID of the result in the Scopus database
2-s2.0-85027352195