Chronic intermittent hypoxia affects the cytosolic phospholipase A(2)alpha/cyclooxygenase 2 pathway via beta(2)-adrenoceptor-mediated ERK/p38 stimulation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F16%3A00466474" target="_blank" >RIV/67985823:_____/16:00466474 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/16:10332874
Result on the web
<a href="http://dx.doi.org/10.1007/s11010-016-2833-8" target="_blank" >http://dx.doi.org/10.1007/s11010-016-2833-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11010-016-2833-8" target="_blank" >10.1007/s11010-016-2833-8</a>
Alternative languages
Result language
angličtina
Original language name
Chronic intermittent hypoxia affects the cytosolic phospholipase A(2)alpha/cyclooxygenase 2 pathway via beta(2)-adrenoceptor-mediated ERK/p38 stimulation
Original language description
Cardiac resistance against acute ischemia/reperfusion (I/R) injury can be enhanced by adaptation to chronic intermittent hypoxia (CIH), but the changes at the molecular level associated with this adaptation are still not fully explored. Phospholipase A(2) (PLA(2)) plays an important role in phospholipid metabolism and may contribute to membrane destruction under conditions of energy deprivation during I/R. The aim of this study was to determine the effect of CIH (7000 m, 8 h/day, 5 weeks) on the expression of cytosolic PLA(2)alpha (cPLA(2)alpha) and its phosphorylated form (p-cPLA(2)alpha), as well as other related signaling proteins in the left ventricular myocardium of adult male Wistar rats. Adaptation to CIH increased the total content of cPLA(2)alpha by 14 % in myocardial homogenate, and enhanced the association of p-cPLA(2)alpha with the nuclear membrane by 85 %. The total number of beta-adrenoceptors (beta-ARs) did not change but the beta(2)/beta(1) ratio markedly increased due to the elevation of beta(2)-ARs and drop in beta(1)-ARs. In parallel, the amount of adenylyl cyclase decreased by 49 % and G(i)alpha proteins increased by about 50 %. Besides that, cyclooxygenase 2 (COX-2) and prostaglandin E-2 (PGE(2)) increased by 36 and 84 %, respectively. In parallel, we detected increased phosphorylation of protein kinase C alpha, ERK1/2 and p38 (by 12, 48 and 19 %, respectively). These data suggest that adaptive changes induced in the myocardium by CIH may include activation of cPLA(2)alpha and COX-2 via beta(2)-AR/G(i)-mediated stimulation of the ERK/p38 pathway.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FA - Cardiovascular diseases including cardio-surgery
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA13-10267S" target="_blank" >GA13-10267S: Cardiac ischemic tolerance of spontaneously hypertensive rats: involvement of mitochondrial genome</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular and Cellular Biochemistry
ISSN
0300-8177
e-ISSN
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Volume of the periodical
423
Issue of the periodical within the volume
1-2
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
151-163
UT code for WoS article
000387365600015
EID of the result in the Scopus database
2-s2.0-84989184220