Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F18%3A00489869" target="_blank" >RIV/67985823:_____/18:00489869 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.matlet.2017.12.004" target="_blank" >http://dx.doi.org/10.1016/j.matlet.2017.12.004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.matlet.2017.12.004" target="_blank" >10.1016/j.matlet.2017.12.004</a>

Result language

angličtina

Original language name

Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

Original language description

Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])

Project

<a href="/en/project/NV15-33018A" target="_blank" >NV15-33018A: Application of adipose tissue-derived stem cells obtained by liposuction in tissue engineering</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Materials Letters

ISSN

0167-577X

e-ISSN

—

Volume of the periodical

214

Issue of the periodical within the volume

Mar 1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

4

Pages from-to

186-189

UT code for WoS article

000419724400048

EID of the result in the Scopus database

2-s2.0-85042236906