Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F18%3A00489871" target="_blank" >RIV/67985823:_____/18:00489871 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3168/jds.2017-13119" target="_blank" >http://dx.doi.org/10.3168/jds.2017-13119</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3168/jds.2017-13119" target="_blank" >10.3168/jds.2017-13119</a>
Alternative languages
Result language
angličtina
Original language name
Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells
Original language description
Recently, milk-derived proteins have attracted attention for applications in the biomedical field such as tissue regeneration. Whey protein isolate (WPI), especially its main component beta-lactoglobulin, can modulate immunity and acts as an antioxidant, antitumor, antiviral, and antibacterial agent. There are very few reports of the application of WPI in tissue engineering, especially in bone tissue engineering. In this study, we tested the influence of different concentrations of WPI on behavior of human osteoblast-like Saos-2 cells, human adipose tissue-derived stem cells (ASC), and human neonatal dermal fibroblasts (FIB ). The positive effect on growth was apparent for Saos-2 cells and FIB but not for ASC. However, the expression of markers characteristic for early osteogenic cell differentiation [type-I collagen (COLT) and alkaline phosphatase (ALP)] as well as ALP activity, increased dose-dependently in ASC. Importantly, Saos-2 cells were able to deposit calcium in the presence of WPI, even in a proliferation medium without other supplements that support osteogenic cell differentiation. The results indicate that, depending on the cell type, WPI can act as an enhancer of cell proliferation and osteogenic differentiation. Therefore, enrichment of biomaterials for bone regeneration with WPI seems a promising approach, especially due to the low cost of WPI.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30404 - Biomaterials (as related to medical implants, devices, sensors)
Result continuities
Project
<a href="/en/project/NV15-33018A" target="_blank" >NV15-33018A: Application of adipose tissue-derived stem cells obtained by liposuction in tissue engineering</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Dairy Science
ISSN
0022-0302
e-ISSN
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Volume of the periodical
101
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
28-36
UT code for WoS article
000418498800003
EID of the result in the Scopus database
2-s2.0-85034433045