beta-Adrenergic signaling, monoamine oxidase A and antioxidant defence in the myocardium of SHR and SHR-mtBN conplastic rat strains: the effect of chronic hypoxia

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F18%3A00490865" target="_blank" >RIV/67985823:_____/18:00490865 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s12576-017-0546-8" target="_blank" >http://dx.doi.org/10.1007/s12576-017-0546-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12576-017-0546-8" target="_blank" >10.1007/s12576-017-0546-8</a>

Result language

angličtina

Original language name

beta-Adrenergic signaling, monoamine oxidase A and antioxidant defence in the myocardium of SHR and SHR-mtBN conplastic rat strains: the effect of chronic hypoxia

Original language description

The beta-adrenergic signaling pathways and antioxidant defence mechanisms play important roles in maintaining proper heart function. Here, we examined the effect of chronic normobaric hypoxia (CNH, 10% O-2, 3 weeks) on myocardial beta-adrenergic signaling and selected components of the antioxidant system in spontaneously hypertensive rats (SHR) and in a conplastic SHR-mtBN strain characterized by the selective replacement of the mitochondrial genome of SHR with that of the more ischemia-resistant Brown Norway strain. Our investigations revealed some intriguing differences between the two strains at the level of beta-adrenergic receptors (beta-ARs), activity of adenylyl cyclase (AC) and monoamine oxidase A (MAO-A), as well as distinct changes after CNH exposure. The beta(2)-AR/beta(1)-AR ratio was significantly higher in SHR-mtBN than in SHR, apparently due to increased expression of beta(2)-ARs. Adaptation to hypoxia elevated beta(2)-ARs in SHR and decreased the total number of beta-ARs in SHR-mtBN. In parallel, the ability of isoprenaline to stimulate AC activity was found to be higher in SHR-mtBN than that in SHR. Interestingly, the activity of MAO-A was notably lower in SHR-mtBN than in SHR, and it was markedly elevated in both strains after exposure to hypoxia. In addition to that, CNH markedly enhanced the expression of catalase and aldehyde dehydrogenase-2 in both strains, and decreased the expression of Cu/Zn superoxide dismutase in SHR. Adaptation to CNH intensified oxidative stress to a similar extent in both strains and elevated the IL-10/TNF-alpha ratio in SHR-mtBN only. These data indicate that alterations in the mitochondrial genome can result in peculiar changes in myocardial beta-adrenergic signaling, MAO-A activity and antioxidant defence and may, thus, affect the adaptive responses to hypoxia.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30105 - Physiology (including cytology)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Physiological Sciences

ISSN

1880-6546

e-ISSN

—

Volume of the periodical

68

Issue of the periodical within the volume

4

Country of publishing house

JP - JAPAN

Number of pages

14

Pages from-to

441-454

UT code for WoS article

000434180700012

EID of the result in the Scopus database

2-s2.0-85020060772