Modulation of NMDA-Mediated Clock Resetting in the Suprachiasmatic Nuclei of mPer2(Luc) Mouse by Endocannabinoids
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00503881" target="_blank" >RIV/67985823:_____/19:00503881 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.3389/fphys.2019.00361" target="_blank" >https://doi.org/10.3389/fphys.2019.00361</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fphys.2019.00361" target="_blank" >10.3389/fphys.2019.00361</a>
Alternative languages
Result language
angličtina
Original language name
Modulation of NMDA-Mediated Clock Resetting in the Suprachiasmatic Nuclei of mPer2(Luc) Mouse by Endocannabinoids
Original language description
Light entrains the master circadian clock in the suprachiasmatic nucleus (SCN) predominantly through glutamatergic signaling via NMDA receptors. The magnitude and the direction of resulting phase shifts depend on timing of the photic stimulus. Previous reports based on behavioral and electrophysiological data suggested that endocannabinoids (EC) might reduce the ability of the SCN clock to respond to light. However, there is little direct evidence for the involvement of EC in entrainment of the rhythmic clock gene expression in the SCN. We have used luminescence recording of cultured SCN slices from mPer2(Luc) mice to construct a complete phase response curve (PRC) for NMDA receptor activation. The results demonstrated that NMDA administration phase-shifts the PER2 rhythm in a time-specific manner. A stable “singularity,” in the course of which the clock seemingly stops while the overall phase is caught between delays and advances, can occur in response to NMDA at a narrow interval during the PER2 level decrease. NMDA-induced phase delays were affected neither by the agonist (WIN 55,212-2 mesylate) nor by the antagonist (rimonabant hydrochloride) of EC receptors. However, the agonist significantly reduced the NMDA-induced phase advance of the clock, while the antagonist enhanced the phase advance, causing a shift in the sensitivity window of the SCN to NMDA. The modulation of EC signaling in the SCN had no effect by itself on the phase of the PER2 rhythm. The results provide evidence for a modulatory role of EC in photic entrainment of the circadian clock in the SCN.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30105 - Physiology (including cytology)
Result continuities
Project
<a href="/en/project/GA17-14704S" target="_blank" >GA17-14704S: The crosstalk between the endocannabinoid and circadian systems</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in physiology
ISSN
1664-042X
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
Mar 29
Country of publishing house
CH - SWITZERLAND
Number of pages
9
Pages from-to
361
UT code for WoS article
000462817500001
EID of the result in the Scopus database
2-s2.0-85066441717