HNK-1 in Morphological Study of Development of the Cardiac Conduction System in Selected Groups of Sauropsida
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00503883" target="_blank" >RIV/67985823:_____/19:00503883 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/19:10396524
Result on the web
<a href="https://doi.org/10.1002/ar.23925" target="_blank" >https://doi.org/10.1002/ar.23925</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/ar.23925" target="_blank" >10.1002/ar.23925</a>
Alternative languages
Result language
angličtina
Original language name
HNK-1 in Morphological Study of Development of the Cardiac Conduction System in Selected Groups of Sauropsida
Original language description
Human natural killer (HNK)-1 antibody is an established marker of developing cardiac conduction system (CCS) in birds and mammals. In our search for the evolutionary origin of the CCS, we tested this antibody in a variety of sauropsid species (Crocodylus niloticus, Varanus indicus, Pogona vitticeps, Pantherophis guttatus, Eublepharis macularius, Gallus gallus, and Coturnix japonica). Hearts of different species were collected at various stages of embryonic development and studied to map immunoreactivity in cardiac tissues. We performed detection on alternating serial paraffin sections using immunohistochemistry for smooth muscle actin or sarcomeric actin as myocardial markers, and HNK-1 to visualize overall staining pattern and then positivity in specific myocyte populations. We observed HNK-1 expression of various intensity distributed in the extracellular matrix and mesenchymal cell surface of cardiac cushions in most of the examined hearts. Strong staining was found in the cardiac nerve fibers and ganglia in all species. The myocardium of the sinus venosus and the atrioventricular canal exhibited transitory patterns of expression. In the Pogona and Crocodylus hearts, as well as in the Gallus and Coturnix ones, additional expression was detected in a subset of myocytes of the (inter)ventricular septum. These results support the use of HNK-1 as a conserved marker of the CCS and suggest that there is a rudimentary CCS present in developing reptilian hearts.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30106 - Anatomy and morphology (plant science to be 1.6)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
ISSN
1932-8486
e-ISSN
—
Volume of the periodical
302
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
69-82
UT code for WoS article
000459881400008
EID of the result in the Scopus database
2-s2.0-85054860686