Mitochondrial Nucleoids: Superresolution microscopy analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00504242" target="_blank" >RIV/67985823:_____/19:00504242 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.biocel.2018.10.012" target="_blank" >https://doi.org/10.1016/j.biocel.2018.10.012</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.biocel.2018.10.012" target="_blank" >10.1016/j.biocel.2018.10.012</a>

Result language
angličtina
Original language name
Mitochondrial Nucleoids: Superresolution microscopy analysis
Original language description
The mitochondrion owns an autonomous genome. Double-stranded circular mitochondrial DNA (mtDNA) is organized in complexes with a packing/stabilizing transcription factor TFAM, having multiple roles, and proteins of gene expression machinery in structures called nucleoids. From hundreds to thousands nucleoids exist distributed in the matrix of mitochondria] reticulum network. A single mtDNA molecule contained within the single nucleoid is a currently preferred but questioned model. Nevertheless, mtDNA replication should lead transiently to its doubling within a nucleoid. However, nucleoid division has not yet been documented in detail. A 3D superresolution microscopy is required to resolve nucleoid biology occurring in similar to 100 nm space, having an advantage over electron microscopy tomography in resolving the particular protein components. We discuss stochastic vs. stimulated emission depletion microscopy yielding wide vs. narrow nucleoid size distribution, respectively. Nucleoid clustering into spheroids fragmented from the continuous mitochondria] network, likewise possible nucleoid attachment to the inner membrane is reviewed.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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