Diversity in Seed Storage Protein Profile of Oilseed Crop Plukenetia volubilis from Peruvian Amazon

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00504502" target="_blank" >RIV/67985823:_____/19:00504502 - isvavai.cz</a>

Alternative codes found

RIV/60460709:41340/19:79360 RIV/00027006:_____/19:00005353

Result on the web

<a href="http://www.fspublishers.org/published_papers/23739_26%20doi%2015.0945%20IJAB-18-1080%20(10)%20679-688.pdf" target="_blank" >http://www.fspublishers.org/published_papers/23739_26%20doi%2015.0945%20IJAB-18-1080%20(10)%20679-688.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Diversity in Seed Storage Protein Profile of Oilseed Crop Plukenetia volubilis from Peruvian Amazon

Original language description

Sacha inchi (Plukenetia volubilis L.) is a plant native to the Peruvian Amazon and produces seeds rich in some nutraceutical compounds with a high protein content. The present work aimed to characterize P. volubilis seed proteins and compare their proteomic profile between different P. volubilis populations. Crude protein content in the seeds was detected at a mean level of 22.56% with the major proportion of albumins and globulins (16.37%), followed by glutelins (5.87%) and a very low content of prolamins (0.33%). Sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel electrophoresis of both total seed flour and Osborne's protein fractions of different populations showed that proteins were concentrated in the 8-75 kDa range. Differences in abundance of proteins between Sacha inchi populations were detected by SD S-PAGE and two-dimensional (2-DE) gel electrophoresis. Gel protein bands and spots of interest were excised, digested with trypsin and analyzed by nano-liquid chromatography/tandem mass spectrometry (nLC-MS/MS). This is the first direct identification of these proteins in actual Sacha inchi seeds. Three proteins (Oleosin 2, Oleosin 3 and elongation factor 1-alpha) directly from P. volubilis and peptides with a sequence identical to 56 proteins from the Euphothiaceae family were detected. This characterization of seed storage protein, their variability within populations and identification of important proteins provide a basis for further investigations for the food industry and bioengineering.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GA15-01948S" target="_blank" >GA15-01948S: Capillary electromigration techniques using affinity selectors & smart polymers for analysis and properties and interactions studies of biomolecules</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Agriculture and Biology

ISSN

1560-8530

e-ISSN

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Volume of the periodical

21

Issue of the periodical within the volume

3

Country of publishing house

PK - PAKISTAN

Number of pages

10

Pages from-to

679-688

UT code for WoS article

000456544000026

EID of the result in the Scopus database

2-s2.0-85062499141