Diurnal expression of ABC and SLC transporters in jejunum is modulated by adrenalectomy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00511931" target="_blank" >RIV/67985823:_____/19:00511931 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/19:10408786

Result on the web

<a href="https://doi.org/10.1016/j.cbpc.2019.108607" target="_blank" >https://doi.org/10.1016/j.cbpc.2019.108607</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cbpc.2019.108607" target="_blank" >10.1016/j.cbpc.2019.108607</a>

Result language

angličtina

Original language name

Diurnal expression of ABC and SLC transporters in jejunum is modulated by adrenalectomy

Original language description

The circadian clock system drives many physiological processes, including plasma concentration of glucocorticoids and epithelial transport of some ions and nutrients. As glucocorticoids entrain the circadian rhythms in various peripheral organs, we examined whether adrenalectomy affects the expression and circadian rhythmicity of intestinal transporters of the solute carrier (SLC) and ATP-binding cassette (ABC) families, which participate in intestinal barriers for absorption of nutrients, nonnutrients and oral drugs. The rat jejunum showed rhythmic circadian profiles of Sglt1, Pept1, Nhe3, Mdr1 and Mrp2 but not Mct1, Oct1, Octn1, Oatp1, Cnt1 and Bcrp. With the exception of Pept1 and Mct1, adrenalectomy decreased the expression of all rhythmic and arrhythmic transporters including the amplitude of Sglt1 and Nhe3 rhythms but minimally affected the phases of rhythmic transporters except of Nhe3. Similarly, adrenalectomy downregulated the expression of rhythmic (Pparα, Hlf, Pgc1α) and arrhythmic (Hnf1β, Hnf4α) transcription factors, which are known to regulate the expression of transporters. We conclude that endogenous corticosteroids have a profound effect on the expression of intestinal SLC and ABC transporters and their nuclear transcription factors. The circulating corticosteroids are necessary for maintaining upregulated expression of Sglt1, Oct1, Octn1, Oatp1, Cnt1, Nhe3, Mdr1, Bcrp, Mrp2, Pparα, Pgc1α, Hnf1β, Hnf4α and Hlf and for maintaining the high amplitude of Sglt1, Nhe3, Pparα, Pgc1α and Hlf circadian rhythms. The study demonstrates that signals from the adrenal gland are necessary for maintaining the expression of arrhythmic and rhythmic intestinal transporters and that changes in the secretion of corticosteroids associated with stress might reorganize intestinal transport barriers.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30105 - Physiology (including cytology)

Project

<a href="/en/project/GA13-08304S" target="_blank" >GA13-08304S: Role of internal time-keeping system in pathogenesis of colorectal cancer</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Comparative Biochemistry and Physiology Part C

ISSN

1532-0456

e-ISSN

—

Volume of the periodical

226

Issue of the periodical within the volume

Dec

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

108607

UT code for WoS article

000496603700003

EID of the result in the Scopus database

2-s2.0-85070900880