Plasma treatment in air at atmospheric pressure that enables reagent-free covalent immobilization of biomolecules on polytetrafluoroethylene (PTFE)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F20%3A00524475" target="_blank" >RIV/67985823:_____/20:00524475 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11320/20:10419299

Result on the web

<a href="https://doi.org/10.1016/j.apsusc.2020.146128" target="_blank" >https://doi.org/10.1016/j.apsusc.2020.146128</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.apsusc.2020.146128" target="_blank" >10.1016/j.apsusc.2020.146128</a>

Result language

angličtina

Original language name

Plasma treatment in air at atmospheric pressure that enables reagent-free covalent immobilization of biomolecules on polytetrafluoroethylene (PTFE)

Original language description

Covalent immobilization of biomolecules to surfaces is desirable in applications in biomedicine and biotechnology, such as biosensors, protein microarrays and implantable biomedical devices. Surface-embedded radicals in polymers, produced by plasma immersion ion implantation, are known to covalently immobilize biomolecules directly from buffer without additional reagents. Here we explore the prospects for creating a surface activated for direct covalent immobilization using a dielectric barrier discharge in air at atmospheric pressure, eliminating the need for vacuum and gas feed systems. We find that a surface activation process at atmospheric pressure in air can activate polytetrafluoroethylene (PTFE) in order to achieve reagent-free covalent immobilization of biomolecules. The presence of surface immobilized protein was verified by X-ray photoelectron spectroscopy (XPS), and its covalent immobilization was demonstrated by resilience to rigorous SDS washing at elevated temperature. Time course immobilization studies show that the covalent coupling capability of the activated surfaces is retained for several days. Proof-of-concept cell assays with immobilized tropoelastin demonstrate the technique’s ability to present functional cell binding molecules for the production of truly bioactive surfaces.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied Surface Science

ISSN

0169-4332

e-ISSN

—

Volume of the periodical

518

Issue of the periodical within the volume

Jul 15

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

146128

UT code for WoS article

000531746300005

EID of the result in the Scopus database

2-s2.0-85082704998