Glucose-Induced Expression of DAPIT in Pancreatic beta-Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F20%3A00531801" target="_blank" >RIV/67985823:_____/20:00531801 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2218-273X/10/7/1026" target="_blank" >https://www.mdpi.com/2218-273X/10/7/1026</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/biom10071026" target="_blank" >10.3390/biom10071026</a>

Result language

angličtina

Original language name

Glucose-Induced Expression of DAPIT in Pancreatic beta-Cells

Original language description

Transcript levels for selected ATP synthase membrane F-O-subunits-including DAPIT-in INS-1E cells were found to be sensitive to lowering glucose down from 11 mM, in which these cells are routinely cultured. Depending on conditions, the diminished mRNA levels recovered when glucose was restored to 11 mM, or were elevated during further 120 min incubations with 20-mM glucose. Asking whether DAPIT expression may be elevated by hyperglycemia in vivo, we studied mice with hyaluronic acid implants delivering glucose for up to 14 days. Such continuous two-week glucose stimulations in mice increased DAPIT mRNA by >5-fold in isolated pancreatic islets (ATP synthase F-1 alpha mRNA by 1.5-fold). In INS-1E cells, the glucose-induced ATP increment vanished with DAPIT silencing (6% of ATP rise), likewise a portion of the mtDNA-copy number increment. With 20 and 11-mM glucose the phosphorylating/non-phosphorylating respiration rate ratio diminished to similar to 70% and 96%, respectively, upon DAPIT silencing, whereas net GSIS rates accounted for 80% and 90% in USMG5/DAPIT-deficient cells. Consequently, the sufficient DAPIT expression and complete ATP synthase assembly is required for maximum ATP synthesis and mitochondrial biogenesis, but not for insulin secretion as such. Elevated DAPIT expression at high glucose further increases the ATP synthesis efficiency.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30202 - Endocrinology and metabolism (including diabetes, hormones)

Project

<a href="/en/project/GA20-00408S" target="_blank" >GA20-00408S: Pancreatic beta cell redox signaling in insulin secretion mechanism and type 2 diabetes etiology</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomolecules

ISSN

2218-273X

e-ISSN

—

Volume of the periodical

10

Issue of the periodical within the volume

7

Country of publishing house

CH - SWITZERLAND

Number of pages

24

Pages from-to

1026

UT code for WoS article

000557063800001

EID of the result in the Scopus database

2-s2.0-85088028462