Communicating macropores in PHEMA-based hydrogels for cell seeding: probabilistic open pore simulation and direct micro-CT proof
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F21%3A00535213" target="_blank" >RIV/67985823:_____/21:00535213 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21220/21:00344096 RIV/61389013:_____/21:00535213 RIV/00216208:11110/21:10421919 RIV/00064165:_____/21:10421919
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0264127520308480?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0264127520308480?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.matdes.2020.109312" target="_blank" >10.1016/j.matdes.2020.109312</a>
Alternative languages
Result language
angličtina
Original language name
Communicating macropores in PHEMA-based hydrogels for cell seeding: probabilistic open pore simulation and direct micro-CT proof
Original language description
Open macroporosity is crucial for scaffolds in tissue engineering. Porogen-templating method is an attractive approach for fabrication of macroporous hydrogels, however, the effect of shape and amount of template particles on imprinted structure has not yet been quantitatively established. We present a mathematical model for simulating the formation of paths percolating through distributed cubical particles as a function of the filling volume. The model was used to select the fraction of NaCl particles as templates for preparation of hydrogels with communicating pores. Hydrogels were prepared from 2-hydroxyethyl methacrylate (HEMA) copolymerized with 2-ethoxyethyl methacrylate (EOEMA), [2-methacryloyloxy)ethyl]trimethylammonium chloride (MOETACl) or ionizable methacrylic acid (MANa) to modulate swelling, surface and mechanical properties of gels. Micro-CT analysis of swollen samples proved a highly-interconnected pore structure. Charged hydrogels swelled more and their apparent elastic modulus G′ was below 1 kPa. For PHEMA and P(HEMA/EOEMA) hydrogels, G′ was 5 and 80 kPa, respectively. Within two-week in vitro studies, MG63 osteoblasts proliferated fastest on P(HEMA/EOEMA) showing the lowest swelling and the highest elastic modulus, whereas cell growth was impaired on positively charged P(HEMA/MOETACl). The mathematical simulation of cubical particle packing in hydrogels and micro-CT data in swollen state provided evidence of an extensive void communication in 3D.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Materials and Design
ISSN
0264-1275
e-ISSN
1873-4197
Volume of the periodical
198
Issue of the periodical within the volume
15 January
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
109312
UT code for WoS article
000607549100007
EID of the result in the Scopus database
2-s2.0-85097233064