Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F21%3A00541894" target="_blank" >RIV/67985823:_____/21:00541894 - isvavai.cz</a>

Alternative codes found

RIV/61389013:_____/21:00541894 RIV/00023001:_____/21:00080770 RIV/68407700:21460/21:00348913

Result on the web

<a href="https://iopscience.iop.org/article/10.1088/1748-605X/abbdbd" target="_blank" >https://iopscience.iop.org/article/10.1088/1748-605X/abbdbd</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1748-605X/abbdbd" target="_blank" >10.1088/1748-605X/abbdbd</a>

Result language

angličtina

Original language name

Accelerated in vitro recellularization of decellularized porcine pericardium for cardiovascular grafts

Original language description

An ideal decellularized allogenic or xenogeneic cardiovascular graft should be capable of preventing thrombus formation after implantation. The antithrombogenicity of the graft is ensured by a confluent endothelial cell layer formed on its surface. Later repopulation and remodeling of the scaffold by the patient's cells should result in the formation of living autologous tissue. In the work presented here, decellularized porcine pericardium scaffolds were modified by growing a fibrin mesh on the surface and inside the scaffolds, and by attaching heparin and human vascular endothelial growth factor (VEGF) to this mesh. Then the scaffolds were seeded with human adipose tissue-derived stem cells (ASCs). While the ASCs grew only on the surface of the decellularized pericardium, the fibrin-modified scaffolds were entirely repopulated in 28 d, and the scaffolds modified with fibrin, heparin and VEGF were already repopulated within 6 d. Label free mass spectrometry revealed fibronectin, collagens, and other extracellular matrix proteins produced by ASCs during recellularization. Thin layers of human umbilical endothelial cells were formed within 4 d after the cells were seeded on the surfaces of the scaffold, which had previously been seeded with ASCs. The results indicate that an artificial tissue prepared by in vitro recellularization and remodeling of decellularized non-autologous pericardium with autologous ASCs seems to be a promising candidate for cardiovascular grafts capable of accelerating in situ endothelialization. ASCs resemble the valve interstitial cells present in heart valves. An advantage of this approach is that ASCs can easily be collected from the patient by liposuction.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30201 - Cardiac and Cardiovascular systems

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Materials

ISSN

1748-6041

e-ISSN

1748-605X

Volume of the periodical

16

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

025024

UT code for WoS article

000621486800001

EID of the result in the Scopus database

2-s2.0-85100632730