ADHESION AND GROWTH OF ADIPOSE TISSUE-DERIVED STEM CELLS ON FIBRIN ASSEMBLIES WITH ATTACHED GROWTH FACTORS FOR TISSUE ENGINEERING OF HEART VALVES

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F19%3A00345868" target="_blank" >RIV/68407700:21460/19:00345868 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/19:00508525 RIV/61389013:_____/19:00508525

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

ADHESION AND GROWTH OF ADIPOSE TISSUE-DERIVED STEM CELLS ON FIBRIN ASSEMBLIES WITH ATTACHED GROWTH FACTORS FOR TISSUE ENGINEERING OF HEART VALVES

Original language description

Currently used xenogeneic biological heart valve prostheses are decellularized and crosslinked with glutaraldehyde. These grafts usually undergo degeneration and calcification. Pericardium-based heart valve prostheses, re-seeded with autologous cells, i.e. adipose tissue-derived cells (ASCs) and endothelial cells, could have longer durability and biocompatibility. In order to improve the adhesion of cells and their ingrowth into decellularized pericardium, various fibrin (Fb) layers were developed, i.e. Fb, Fb with covalently bound heparin (H), Fb with either vascular endothelial growth factor (VEGF) or fibroblast growth factor 2 (FGF) in various concentrations (1 ng/ml, 10 ng/ml, 100 ng/ml) or with both VEGF and FGF (100 ng/ml). Growth factors were attached onto Fb via heparin or were adsorbed. ASCs were seeded on theses layers in a DMEM medium supplemented with 2 % of fetal bovine serum, TGF beta 1 and BMP-4 (both 2.5 ng/ml), and with ascorbic acid. Cell adhesion and growth/viability was assessed by counted cell number/MTS evaluation. ASCs were stained for differentiation markers of smooth muscle cells, such as alpha-actin, calponin, and myosin heavy chain. On day 7, ASCs on Fb_H_VEGF layers produced both calponin and alpha-actin. An increased FGF concentration caused reduced calponin staining of ASCs. Lack of heparin in fibrin assemblies with growth factors inhibited the production of both alpha-actin and calponin in ASCs. The highest ASCs density/viability was found on Fb_H_VEGF_FGF layer. The proper formulation of fibrin coatings could be favorable for ASCs growth and differentiation and could subsequently support endothelialization of cardiovascular prostheses with endothelial cells.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

30201 - Cardiac and Cardiovascular systems

Project

<a href="/en/project/NV15-29153A" target="_blank" >NV15-29153A: Development of an aortic heart valve based on pericardium using primary and stem cells and mechanical loading in a bioreactor</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

10TH ANNIVERSARY INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH & APPLICATION (NANOCON 2018)

ISBN

978-80-87294-89-5

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

324-329

Publisher name

TANGER

Place of publication

Ostrava

Event location

Brno

Event date

Oct 17, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000513131900056