Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F21%3A00543905" target="_blank" >RIV/67985823:_____/21:00543905 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s10482-021-01578-8" target="_blank" >https://doi.org/10.1007/s10482-021-01578-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10482-021-01578-8" target="_blank" >10.1007/s10482-021-01578-8</a>
Alternative languages
Result language
angličtina
Original language name
Anhydrobiosis in yeast: role of cortical endoplasmic reticulum protein Ist2 in Saccharomyces cerevisiae cells during dehydration and subsequent rehydration
Original language description
Two Saccharomyces cerevisiae strains, BY4741 and BY4741-derived strain lacking the IST2 gene (ist2 Delta), were used to characterise the possible role of cortical endoplasmic reticulum (ER) protein Ist2 upon cell dehydration and subsequent rehydration. For the first time, we show that not only protein components of the plasma membrane (PM), but also at least one ER membrane protein (Ist2) play an important role in the maintenance of the viability of yeast cells during dehydration and subsequent rehydration. The low viability of the mutant strain ist2 increment upon dehydration-rehydration stress was related to the lack of Ist2 protein in the ER. We revealed that the PM of ist2 increment strain is not able to completely restore its molecular organisation during reactivation from the state of anhydrobiosis. As the result, the permeability of the PM remains high regardless of the type of reactivation (rapid or gradual rehydration). We conclude that ER protein Ist2 plays an important role in ensuring the stability of molecular organisation and functionality of the PM during dehydration-rehydration stress. These results indicate an important role of ER-PM interactions during cells transition into the state of anhydrobiosis and the subsequent restoration of their physiological activities.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/LTC20006" target="_blank" >LTC20006: Transport systems involved in intracellular pH homeostasis and yeast tolerance to low external pH</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology
ISSN
0003-6072
e-ISSN
1572-9699
Volume of the periodical
114
Issue of the periodical within the volume
7
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
1069-1077
UT code for WoS article
000639508200002
EID of the result in the Scopus database
2-s2.0-85104409085