Influence of Biomimetically Mineralized Collagen Scaffolds on Bone Cell Proliferation and Immune Activation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F22%3A00555930" target="_blank" >RIV/67985823:_____/22:00555930 - isvavai.cz</a>
Alternative codes found
RIV/00027022:_____/22:N0000072 RIV/60461373:22310/22:43924596
Result on the web
<a href="https://www.mdpi.com/2073-4360/14/3/602" target="_blank" >https://www.mdpi.com/2073-4360/14/3/602</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/polym14030602" target="_blank" >10.3390/polym14030602</a>
Alternative languages
Result language
angličtina
Original language name
Influence of Biomimetically Mineralized Collagen Scaffolds on Bone Cell Proliferation and Immune Activation
Original language description
Collagen, as the main component of connective tissue, is frequently used in various tissue engineering applications. In this study, porous sponge-like collagen scaffolds were prepared by freeze-drying and were then mineralized in a simulated body fluid. The mechanical stability was similar in both types of scaffolds, but the mineralized scaffolds (MCS) contained significantly more calcium, magnesium and phosphorus than the unmineralized scaffolds (UCS). Although the MCS contained a lower percentage (~32.5%) of pores suitable for cell ingrowth (113-357 mu m in diameter) than the UCS (~70%), the number of human-osteoblast-like MG-63 cells on days 1, 3 and 7 after seeding was higher on MCS than on UCS, and the cells penetrated deeper into the MCS. The cell growth in extracts prepared by eluting the scaffolds for 7 days in a cell culture medium was also markedly higher in the MCS extracts, as indicated by real-time monitoring in the sensory xCELLigence system for 7 days. From this point of view, MCS are more promising for bone tissue engineering than UCS. However, MCS evoked a more pronounced inflammatory response than UCS, as indicated by the production of tumor necrosis factor-alpha (TNF-alpha) in macrophage-like RAW 264.7 cells in cultures on these scaffolds.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30404 - Biomaterials (as related to medical implants, devices, sensors)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Polymers
ISSN
2073-4360
e-ISSN
2073-4360
Volume of the periodical
14
Issue of the periodical within the volume
3
Country of publishing house
CH - SWITZERLAND
Number of pages
26
Pages from-to
602
UT code for WoS article
000756296200001
EID of the result in the Scopus database
2-s2.0-85124190525