Polylactide nanofibers with hydroxyapatite as growth substrates for osteoblast-like cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F14%3A00316032" target="_blank" >RIV/68407700:21220/14:00316032 - isvavai.cz</a>

Alternative codes found

RIV/68378271:_____/14:00434268 RIV/67985823:_____/14:00434268 RIV/61389013:_____/14:00434268 RIV/67985891:_____/14:00434268

Result on the web

<a href="http://dx.doi.org/10.1002/jbm.a.35061" target="_blank" >http://dx.doi.org/10.1002/jbm.a.35061</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jbm.a.35061" target="_blank" >10.1002/jbm.a.35061</a>

Result language

angličtina

Original language name

Polylactide nanofibers with hydroxyapatite as growth substrates for osteoblast-like cells

Original language description

Various types of nanofibers are increasingly used in tissue engineering, mainly for their ability to mimic the architecture of tissue at the nanoscale. We evaluated the adhesion, growth, viability, and differentiation of human osteoblast-like MG 63 cells on polylactide (PLA) nanofibers prepared by needle-less electrospinning and loaded with 5 or 15 wt % of hydroxyapatite (HA) nanoparticles. On day 7 after seeding, the cell number was the highest on samples with 15 wt % of HA. This result was confirmed by the XTT test, especially after dynamic cultivation, when the number of metabolically active cells on these samples was even higher than on control polystyrene. Staining with a live/dead kit showed that the viability of cells on all nanofibrous scaffolds was very high and comparable to that on control polystyrene dishes. An enzyme-linked immunosorbent assay revealed that the concentration of osteocalcin was also higher in cells on samples with 15 wt % of HA. There was no immune activation of cells (measured by production of TNF-alpha), associated with the incorporation of HA. Moreover, the addition of HA suppressed the creep behavior of the scaffolds in their dry state. Thus, nanofibrous PLA scaffolds have potential for bone tissue engineering, particularly those with 15 wt % of HA. (C) 2013 Wiley Periodicals, Inc.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

21001 - Nano-materials (production and properties)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biomedical Materials Research Part A

ISSN

1549-3296

e-ISSN

1552-4965

Volume of the periodical

2014

Issue of the periodical within the volume

102/11

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

3918-3930

UT code for WoS article

000343010100016

EID of the result in the Scopus database

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