The possible role of hypoxia in the affected tissue of relapsed clubfoot
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F22%3A00556129" target="_blank" >RIV/67985823:_____/22:00556129 - isvavai.cz</a>
Alternative codes found
RIV/44555601:13450/22:43897303 RIV/00216208:11110/22:10441997 RIV/00216208:11130/22:10441997 RIV/00064211:_____/22:W0000015 RIV/00216208:11510/22:10441997
Result on the web
<a href="https://doi.org/10.1038/s41598-022-08519-z" target="_blank" >https://doi.org/10.1038/s41598-022-08519-z</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-022-08519-z" target="_blank" >10.1038/s41598-022-08519-z</a>
Alternative languages
Result language
angličtina
Original language name
The possible role of hypoxia in the affected tissue of relapsed clubfoot
Original language description
Our aim was to study the expression of hypoxia-related proteins as a possible regulatory pathway in the contracted side tissue of relapsed clubfoot. We compared the expression of hypoxia-related proteins in the tissue of the contracted (medial) side of relapsed clubfoot, and in the tissue of the non-contracted (lateral) side of relapsed clubfoot. Tissue samples from ten patients were analyzed by immunohistochemistry and image analysis, Real-time PCR and Mass Spectrometry to evaluate the differences in protein composition and gene expression. We found a significant increase in the levels of smooth muscle actin, transforming growth factor-beta, hypoxia-inducible factor 1 alpha, lysyl oxidase, lysyl oxidase-like 2, tenascin C, matrix metalloproteinase-2, matrix metalloproteinase-9, fibronectin, collagen types III and VI, hemoglobin subunit alpha and hemoglobin subunit beta, and an overexpression of ACTA2, FN1, TGFB1, HIF1A and MMP2 genes in the contracted medial side tissue of clubfoot. In the affected tissue, we have identified an increase in the level of hypoxia-related proteins, together with an overexpression of corresponding genes. Our results suggest that the hypoxia-associated pathway is potentially a factor contributing to the etiology of clubfoot relapses, as it stimulates both angioproliferation and fibroproliferation, which are considered to be key factors in the progression and development of relapses.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30211 - Orthopaedics
Result continuities
Project
<a href="/en/project/NV17-31564A" target="_blank" >NV17-31564A: Improvement of non-invasive fibrosis treatment of congenital pes equinovarus.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
12
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
4462
UT code for WoS article
000769466200014
EID of the result in the Scopus database
2-s2.0-85126640231