Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F19%3A00504465" target="_blank" >RIV/67985823:_____/19:00504465 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/19:10391092 RIV/00216208:11130/19:10391092 RIV/00216208:11510/19:10391092 RIV/00216208:11310/19:10391092 and 2 more

Result on the web

<a href="https://doi.org/10.1002/jor.24211" target="_blank" >https://doi.org/10.1002/jor.24211</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jor.24211" target="_blank" >10.1002/jor.24211</a>

Result language

angličtina

Original language name

Novel contribution to clubfoot pathogenesis: The possible role of extracellular matrix proteins

Original language description

Idiopathic pes equinovarus (clubfoot) is a congenital deformity of the feet and lower legs. Clubfoot belongs to a group of fibro‐proliferative disorders but its origin remains unknown. Our study aimed to achieve the first complex proteomic comparison of clubfoot contracted tissue of the foot (medial side, n = 16), with non‐contracted tissue (lateral side, n = 13). We used label‐free mass spectrometry quantification and immunohistochemistry. Seven proteins were observed to be significantly upregulated in the medial side (asporin, collagen type III, V, and VI, versican, tenascin‐C, and transforming growth factor beta induced protein) and four in the lateral side (collagen types XII and XIV, fibromodulin, and cartilage intermediate layer protein 2) of the clubfoot. Comparison of control samples from cadavers brought only two different protein concentrations (collagen types I and VI). We also revealed pathological calcification and intracellular positivity of transforming growth factor beta only in the contracted tissue of clubfoot. Most of the 11 differently expressed proteins are strongly related to the extracellular matrix architecture and we assume that they may play specific roles in the pathogenesis of this deformity. These proteins seem to be promising targets for future investigations and treatment of this disease.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30211 - Orthopaedics

Project

<a href="/en/project/NV17-31564A" target="_blank" >NV17-31564A: Improvement of non-invasive fibrosis treatment of congenital pes equinovarus.</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Orthopaedic Research

ISSN

0736-0266

e-ISSN

—

Volume of the periodical

37

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

769-778

UT code for WoS article

000464419100026

EID of the result in the Scopus database

2-s2.0-85061451671