Biomimetic biphasic curdlan-based scaffold for osteochondral tissue engineering applications Characterization and preliminary evaluation of mesenchymal stem cell response in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F22%3A00558807" target="_blank" >RIV/67985823:_____/22:00558807 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.bioadv.2022.212724" target="_blank" >https://doi.org/10.1016/j.bioadv.2022.212724</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioadv.2022.212724" target="_blank" >10.1016/j.bioadv.2022.212724</a>

Result language

angličtina

Original language name

Biomimetic biphasic curdlan-based scaffold for osteochondral tissue engineering applications Characterization and preliminary evaluation of mesenchymal stem cell response in vitro

Original language description

Osteochondral defects remain a huge problem in medicine today. Biomimetic bi- or multi-phasic scaffolds constitute a very promising alternative to osteochondral autografts and allografts. In this study, a new curdlanbased scaffold was designed for osteochondral tissue engineering applications. To achieve biomimetic properties, it was enriched with a protein component ??? whey protein isolate as well as a ceramic ingredient ??? hydroxyapatite granules. The scaffold was fabricated via a simple and cost-efficient method, which represents a significant advantage. Importantly, this technique allowed generation of a scaffold with two distinct, but integrated phases. Scanning electron microcopy and optical profilometry observations demonstrated that phases of biomaterial possessed different structural properties. The top layer of the biomaterial (mimicking the cartilage) was smoother than the bottom one (mimicking the subchondral bone), which is beneficial from a biological point of view because unlike bone, cartilage is a smooth tissue. Moreover, mechanical testing showed that the top layer of the biomaterial had mechanical properties close to those of natural cartilage. Although the mechanical properties of the bottom layer of scaffold were lower than those of the subchondral bone, it was still higher than in many analogous systems. Most importantly, cell culture experiments indicated that the biomaterial possessed high cytocompatibility towards adipose tissue-derived mesenchymal stem cells and bone marrow-derived mesenchymal stem cells in vitro. Both phases of the scaffold enhanced cell adhesion, proliferation, and chondrogenic differentiation of stem cells (revealing its chondroinductive properties in vitro) as well as osteogenic differentiation of these cells (revealing its osteoinductive properties in vitro). Given all features of the novel curdlan-based scaffold, it is worth noting that it may be considered as promising candidate for osteochondral tissue engineering applications.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30404 - Biomaterials (as related to medical implants, devices, sensors)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomaterials Advances

ISSN

2772-9508

e-ISSN

2772-9508

Volume of the periodical

135

Issue of the periodical within the volume

April

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

19

Pages from-to

212724

UT code for WoS article

000812231700001

EID of the result in the Scopus database

2-s2.0-85131954631