Mineralised polylactide and polycaprolactone soft foams with hierarchical micro-macro porous structure for tissue engineering
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F22%3A00567467" target="_blank" >RIV/67985823:_____/22:00567467 - isvavai.cz</a>
Alternative codes found
RIV/00027022:_____/21:N0000009 RIV/67985823:_____/21:00567467
Result on the web
<a href="https://www.confer.cz/nanocon/2021/read/4346-mineralised-polylactide-and-polycaprolactone-soft-foams-with-hierarchical-micro-macro-porous-structure-for-tissue-engineering.pdf" target="_blank" >https://www.confer.cz/nanocon/2021/read/4346-mineralised-polylactide-and-polycaprolactone-soft-foams-with-hierarchical-micro-macro-porous-structure-for-tissue-engineering.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.37904/nanocon.2021.4346" target="_blank" >10.37904/nanocon.2021.4346</a>
Alternative languages
Result language
angličtina
Original language name
Mineralised polylactide and polycaprolactone soft foams with hierarchical micro-macro porous structure for tissue engineering
Original language description
The purpose of the study was development of soft foams from resorbable polymers with unique micro-macro porous interconnected hierarchical structure specially designed as scaffold for engineering of soft tissues. The foams have been prepared by freeze-drying of solutions of polylactide (PLA) and polycaprolactone (PCL) in 1,4-dioxane. The foams prepared by freeze-drying had interconnected porous aerogel characteristics. The hierarchical structure with bimodal micro-macro pore size distribution were obtained after addition of sucrose or NaCl porogens with defined crystal size distributions to the solutions before freeze-drying and leaching the porogen crystals from the freeze-dried foams with demineralised water. Polyethyleneimine was chemically conjugated to the alkali-treated foams followed by conjugation of citric acid using carbodiimide chemistry. Finally, they were mineralised by immersing and incubating in a simulated body fluid with ionic concentration similar to that of human blood plasma, to obtain tissue engineering scaffolds. To verify their biocompatibility, the scaffolds were seeded with adipose-derived stem cells (ASC) and sarcoma osteogenic-2 (SaOs-2) human osteoblast-like cells. Morphology of the cells attached to the scaffolds was evaluated and their viability was verified by a metabolic test. Biocompatibility and usability of the scaffolds was successfully verified by incubation with adipose-derived stem cells and SaOs-2 human osteosarcoma cell line. Mineralised scaffolds are more suitable growth supports for both the cell types than unmineralized collagen scaffolds. The scaffolds have been specially designed for engineering of soft tissues, but they can be used in other categories of tissue engineering, too.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
30404 - Biomaterials (as related to medical implants, devices, sensors)
Result continuities
Project
<a href="/en/project/NU20-08-00208" target="_blank" >NU20-08-00208: Novel vascularized stem-cell based constructs for soft and hard tissue engineering</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
NANOCON 2021 - Conference proceedings
ISBN
978-80-88365-00-6
ISSN
2694-930X
e-ISSN
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Number of pages
6
Pages from-to
298-303
Publisher name
Tanger Ltd.
Place of publication
Ostrava
Event location
Brno
Event date
Oct 20, 2021
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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