Timed Sequence Task: A New Paradigm to Study Motor Learning and Flexibility in Mice

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F23%3A00580217" target="_blank" >RIV/67985823:_____/23:00580217 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1523/ENEURO.0145-23.2023" target="_blank" >https://doi.org/10.1523/ENEURO.0145-23.2023</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1523/ENEURO.0145-23.2023" target="_blank" >10.1523/ENEURO.0145-23.2023</a>

Result language

angličtina

Original language name

Timed Sequence Task: A New Paradigm to Study Motor Learning and Flexibility in Mice

Original language description

Motor learning and flexibility allow animals to perform routine actions efficiently while keeping them flexible. A number of paradigms are used to test cognitive flexibility, but not many of them focus specifically on the learning of complex motor sequences and their flexibility. While many tests use operant or touchscreen boxes that offer high throughput and reproducibility, the motor actions themselves are mostly simple presses of a designated lever. To focus more on motor actions during the operant task and to probe the flexibility of these well trained actions, we developed a new operant paradigm for mice, the „timed sequence task.“ The task re-quires mice to learn a sequence of lever presses that have to be emitted in precisely defined time limits. After training, the required pressing sequence and/or timing of individual presses is modified to test the ability of mice to alter their previously trained motor actions. We provide a code for the new protocol that can be used and adapted to common types of operant boxes. In addition, we provide a set of scripts that allow automatic extraction and analysis of numerous parameters recorded during each session. We demonstrate that the analysis of multiple performance parameters is necessary for detailed insight into the behavior of animals during the task. We validate our paradigm in an experiment using the valproate model of autism as a model of cognitive inflexibility. We show that the valproate mice show superior performance at specific stages of the task, paradoxically because of their propensity to more stereotypic behavior.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

<a href="/en/project/GJ19-07983Y" target="_blank" >GJ19-07983Y: Cholinergic control of striatal-based behavior: determining the role of nicotinic acetylcholine receptors expressed by striatal GABAergic interneurons</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ENEURO

ISSN

2373-2822

e-ISSN

2373-2822

Volume of the periodical

10

Issue of the periodical within the volume

10

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

1-13

UT code for WoS article

001123022300001

EID of the result in the Scopus database

2-s2.0-85173790837