Circadian clock in choroid plexus is resistant to immune challenge but dampens in response to chronodisruption

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F24%3A00583226" target="_blank" >RIV/67985823:_____/24:00583226 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1016/j.bbi.2024.01.217" target="_blank" >https://doi.org/10.1016/j.bbi.2024.01.217</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbi.2024.01.217" target="_blank" >10.1016/j.bbi.2024.01.217</a>

Result language

angličtina

Original language name

Circadian clock in choroid plexus is resistant to immune challenge but dampens in response to chronodisruption

Original language description

The choroid plexus (ChP) in the brain ventricles has a major influence on brain homeostasis. In this study, we aimed to determine whether the circadian clock located in ChP is affected by chronodisruption caused by misalignment with the external light/dark cycle and/or inflammation. Adult mPer2Luc mice were maintained in the LD12:12 cycle or exposed to one of two models of chronic chronodisruption – constant light for 22–25 weeks (cLL) or 6-hour phase advances of the LD12:12 cycle repeated weekly for 12 weeks (cLD-shifts). Locomotor activity was monitored before the 4th ventricle ChP and suprachiasmatic nuclei (SCN) explants were recorded in real time for PER2-driven population and single-cell bioluminescence rhythms. In addition, plasma immune marker concentrations and gene expression in ChP, prefrontal cortex, hippocampus and cerebellum were analyzed. cLL dampened the SCN clock but did not shorten the inactivity interval (sleep). cLD-shifts had no effect on the SCN clock, but transiently affected sleep duration and fragmentation. Both chronodisruption protocols dampened the ChP clock. Although immune markers were elevated in plasma and hippocampus, levels in ChP were unaffected, and unlike the liver clock, the ChP clock was resistant to lipopolysaccharide treatment. Importantly, both chronodisruption protocols reduced glucocorticoid signaling in ChP. The data demonstrate the high resistance of the ChP clock to inflammation, highlighting its role in protecting the brain from neuroinflammation, and on the other hand its high sensitivity to chronodisruption. Our results provide a novel link between human lifestyle-induced chronodisruption and the impairment of ChP-dependent brain homeostasis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

<a href="/en/project/GA21-09745S" target="_blank" >GA21-09745S: Circadian clock in choroid plexus and its sensitivity to chronodisruption and neuroinflammation</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Brain Behavior and Immunity

ISSN

0889-1591

e-ISSN

1090-2139

Volume of the periodical

117

Issue of the periodical within the volume

March

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

15

Pages from-to

255-269

UT code for WoS article

001174463800001

EID of the result in the Scopus database

2-s2.0-85183961992