Biofouling and performance of boron-doped diamond electrodes for detection of dopamine and serotonin in neuron cultivation media

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F24%3A00586010" target="_blank" >RIV/67985823:_____/24:00586010 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/24:10488744

Result on the web

<a href="https://doi.org/10.1016/j.bioelechem.2024.108713" target="_blank" >https://doi.org/10.1016/j.bioelechem.2024.108713</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioelechem.2024.108713" target="_blank" >10.1016/j.bioelechem.2024.108713</a>

Result language

angličtina

Original language name

Biofouling and performance of boron-doped diamond electrodes for detection of dopamine and serotonin in neuron cultivation media

Original language description

Boron doped diamond has been considered as a fouling-resistive electrode material for in vitro and in vivo detection of neurotransmitters. In this study, its performance in electrochemical detection of dopamine and serotonin in neuron cultivation media Neurobasal™ before and after cultivation of rat neurons was investigated. For differential pulse voltammetry the limits of detection in neat Neurobasal™ medium of 2 µM and 0.2 µM for dopamine and serotonin, respectively, were achieved on the polished surface, which is comparable with physiological values. On oxidized surface twofold higher values, but increased repeatabilities of the signals were obtained. However, in Neurobasal™ media with peptides-containing supplements necessary for cell cultivation, the voltammograms were notably worse shaped due to biofouling, especially in the medium isolated after neuron growth. In these complex media, the amperometric detection mode at +0.75 V (vs. Ag/AgCl) allowed to detect portion-wise additions of dopamine and serotonin (as low as 1–2 µM), mimicking neurotransmitter release from vesicles despite the lower sensitivity in comparison with neat NeurobasalTM. The results indicate substantial differences in detection on boron doped diamond electrode in the presence and absence of proteins, and the necessity of studies in real media for successful implementation to neuron-electrode interfaces.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30103 - Neurosciences (including psychophysiology)

Project

<a href="/en/project/GA23-05688S" target="_blank" >GA23-05688S: Electrochemical sensors based on nanostructured and functionalized sp2 and sp3 carbon surfaces for (bio)analytical applications</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioelectrochemistry

ISSN

1567-5394

e-ISSN

1878-562X

Volume of the periodical

158

Issue of the periodical within the volume

August

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

10

Pages from-to

108713

UT code for WoS article

001236414600001

EID of the result in the Scopus database

2-s2.0-85191416681