Beyond glucose: The crucial role of redox signaling in β-cell metabolic adaptation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F24%3A00599348" target="_blank" >RIV/67985823:_____/24:00599348 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/24:00599348 RIV/00216208:11110/24:10486281
Result on the web
<a href="https://doi.org/10.1016/j.metabol.2024.156027" target="_blank" >https://doi.org/10.1016/j.metabol.2024.156027</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.metabol.2024.156027" target="_blank" >10.1016/j.metabol.2024.156027</a>
Alternative languages
Result language
angličtina
Original language name
Beyond glucose: The crucial role of redox signaling in β-cell metabolic adaptation
Original language description
Objective:Redox signaling mediated by reversible oxidative cysteine thiol modifications is crucial for driving cellular adaptation to dynamic environmental changes, maintaining homeostasis, and ensuring proper function. This is particularly critical in pancreatic β-cells, which are highly metabolically active and play a specialized role in whole organism glucose homeostasis. Glucose stimulation in β-cells triggers signals leading to insulin secretion, including changes in ATP/ADP ratio and intracellular calcium levels. Additionally, lipid metabolism and reactive oxygen species (ROS) signaling are essential for β-cell function and health.Methods:We employed IodoTMT isobaric labeling combined with tandem mass spectrometry to elucidate redox signaling pathways in pancreatic β-cells.Results:Glucose stimulation significantly increases ROS levels in β-cells, leading to targeted reversible oxidation of proteins involved in key metabolic pathways such as glycolysis, the tricarboxylic acid (TCA) cycle, pyruvate metabolism, oxidative phosphorylation, protein processing in the endoplasmic reticulum (ER), and insulin secretion. Furthermore, the glucose-induced increase in reversible cysteine oxidation correlates with the presence of other post-translational modifications, including acetylation and phosphorylation.Conclusions:Proper functioning of pancreatic β-cell metabolism relies on fine-tuned regulation, achieved through a sophisticated system of diverse post-translational modifications that modulate protein functions. Our findings demonstrate that glucose induces the production of ROS in pancreatic β-cells, leading to targeted reversible oxidative modifications of proteins. Furthermore, protein activity is modulated by acetylation and phosphorylation, highlighting the complexity of the regulatory mechanisms in β-cell function.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Metabolism-Clinical and Experimental
ISSN
0026-0495
e-ISSN
1532-8600
Volume of the periodical
161
Issue of the periodical within the volume
December
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
156027
UT code for WoS article
001322756200001
EID of the result in the Scopus database
2-s2.0-85204777089