Peptide-coated DNA nanostructures as a platform for control of lysosomal function in cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F24%3A00599407" target="_blank" >RIV/67985823:_____/24:00599407 - isvavai.cz</a>

Alternative codes found

RIV/68378271:_____/24:00599407 RIV/68378041:_____/24:00602810 RIV/00216208:11320/24:10484741 RIV/00023001:_____/24:00085117

Result on the web

<a href="https://doi.org/10.1016/j.cej.2024.155633" target="_blank" >https://doi.org/10.1016/j.cej.2024.155633</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cej.2024.155633" target="_blank" >10.1016/j.cej.2024.155633</a>

Result language

angličtina

Original language name

Peptide-coated DNA nanostructures as a platform for control of lysosomal function in cells

Original language description

DNA nanotechnology is a rapidly growing field that provides exciting tools for biomedical applications. Targeting lysosomal functions with nanomaterials, such as DNA nanostructures (DNs), represents a rational and systematic way to control cell functionality. Here we present a versatile DNA nanostructure-based platform that can modulate a number of cellular functions depending on the concentration and surface decoration of the nanostructure. Utilizing different peptides for surface functionalization of DNs, we were able to rationally modulate lysosomal activity, which in turn translated into the control of cellular function, ranging from changes in cell morphology to modulation of immune signaling and cell death. Low concentrations of decalysine peptidecoated DNs induced lysosomal acidification, altering the metabolic activity of susceptible cells. In contrast, DNs coated with an aurein-bearing peptide promoted lysosomal alkalization, triggering STING activation. High concentrations of decalysine peptide-coated DNs caused lysosomal swelling, loss of cell–cell contacts, and morphological changes without inducing cell death. Conversely, high concentrations of aurein-coated DNs led to lysosomal rupture and mitochondrial damage, resulting in significant cytotoxicity. Our study holds promise for the rational design of a new generation of versatile DNA-based nanoplatforms that can be used in various biomedical applications, like the development of combinatorial anti-cancer platforms, efficient systems for endolysosomal escape, and nanoplatforms modulating lysosomal pH.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30404 - Biomaterials (as related to medical implants, devices, sensors)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical Engineering Journal

ISSN

1385-8947

e-ISSN

1873-3212

Volume of the periodical

498

Issue of the periodical within the volume

Oct

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

25

Pages from-to

155633

UT code for WoS article

001316778900001

EID of the result in the Scopus database

2-s2.0-85203640190