Ultra-inert lanthanide chelates as mass tags for multiplexed bioanalysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F24%3A00601200" target="_blank" >RIV/67985823:_____/24:00601200 - isvavai.cz</a>
Alternative codes found
RIV/61388980:_____/24:00601172 RIV/61388963:_____/24:00601172 RIV/60461373:22340/24:43930076
Result on the web
<a href="https://doi.org/10.1038/s41467-024-53867-1" target="_blank" >https://doi.org/10.1038/s41467-024-53867-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41467-024-53867-1" target="_blank" >10.1038/s41467-024-53867-1</a>

Result language
angličtina
Original language name
Ultra-inert lanthanide chelates as mass tags for multiplexed bioanalysis
Original language description
Coordination compounds of lanthanides are indispensable in biomedical applications as MRI contrast agents and radiotherapeutics. However, since the introduction of the chelator DOTA four decades ago, there has been only limited progress on improving their thermodynamic stability and kinetic inertness, which are essential for safe in vivo use. Here, we present ClickZip, an innovative synthetic strategy employing a coordination-templated formation of a 1,5-triazole bridge that improves kinetic inertness up to a million-fold relative to DOTA, expanding utility of lanthanide chelates beyond traditional uses. Acting as unique mass tags, the ClickZip chelates can be released from (biological) samples by acidic hydrolysis, chromatographically distinguished from interfering lanthanide species, and sensitively detected by mass spectrometry. Lanthanides enclosed in ClickZip chelates are chemically almost indistinguishable, providing a more versatile alternative to chemically identical isotopic labels for multiplexed analysis. The bioanalytical potential is demonstrated on tagged cell-penetrating peptides in vitro, and anti-obesity prolactin-releasing peptides in vivo.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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