Cellulases and their promising use in tissue engineering and cell sheet technology
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F25%3A00636207" target="_blank" >RIV/67985823:_____/25:00636207 - isvavai.cz</a>
Alternative codes found
RIV/68378271:_____/25:00636207 RIV/60461373:22310/25:43933102
Result on the web
<a href="https://doi.org/10.1016/j.matdes.2025.114111" target="_blank" >https://doi.org/10.1016/j.matdes.2025.114111</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.matdes.2025.114111" target="_blank" >10.1016/j.matdes.2025.114111</a>
Alternative languages
Result language
angličtina
Original language name
Cellulases and their promising use in tissue engineering and cell sheet technology
Original language description
Bacterial nanocellulose (BNC) was used to generate continuous scaffold-free sheets of human keratinocytes (HaCaT) after degradation by cellulase from Trichoderma reesei. First, we successfully grafted acidic cellulase from Trichoderma reesei onto a BNC nanostructure and prepared degradable BNC that dissolved in phosphate-buffered saline within one week. In addition, we selected a cellulase solution that degrades BNC within three hours under physiological conditions. HaCaTs were cultured on four different BNC samples: air-dried (BNC_AD), lyophilized (BNC_L) and plasma-modified (PM) BNC exposed to a direct current Ar+plasma discharge (BNC_AD_PM and BNC_L_PM). On days 1 and 3, the cells were stained with the LIVE/DEAD viability/cytotoxicity kit. The significantly highest cell number on day 3 was found on the BNC_AD_PM membrane (median 65,025 cells/cm2), similar to the standard cell culture polystyrene (median 58,553 cells/cm2). Cell viability on day 3 was greater than 90 % for all samples except BNC_L (62 %). Cell island formation was also preferentially observed on BNC_AD_PM samples. The significant positive effect of PM on cell growth was shown after BNC degradation on day 10 after cell seeding, when continuous sheets of viable keratinocytes were separated from the BNC samples by exposure to a cellulase solution (37.5 U/ml, pH 6.5) for 3 h. The number of cells obtained by cultivation on BNC_PM greatly exceeded the number of cells obtained from unmodified BNC. The approaches presented here may be useful for the preparation of (1) degradable BNC scaffolds for tissue engineering and (2) continuous scaffold-free cell sheets applicable in wound healing.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Materials and Design
ISSN
0264-1275
e-ISSN
1873-4197
Volume of the periodical
254
Issue of the periodical within the volume
June
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
114111
UT code for WoS article
001502161100002
EID of the result in the Scopus database
2-s2.0-105006742249