Biocompatibility of Ar plasma-treated fluorinated ethylene propylene: Adhesion and viability of human keratinocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F19%3A43918503" target="_blank" >RIV/60461373:22310/19:43918503 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/19:43918503
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0928493118332521" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0928493118332521</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.msec.2019.02.100" target="_blank" >10.1016/j.msec.2019.02.100</a>
Alternative languages
Result language
angličtina
Original language name
Biocompatibility of Ar plasma-treated fluorinated ethylene propylene: Adhesion and viability of human keratinocytes
Original language description
Suitable surface modification of synthetic polymers is vital for improvement of cell adhesion to such materials. To that effect, reproducible modification methods such as argon plasma treatment are advantageous. We have previously described a significant improvement of cell adhesion and proliferation on Ar-plasma modified fluorinated ethylene propylene (FEP). In the experiments presented here, we sought to gain a closer insight into cell adhesion on modified FEP matrices by means of analysis of focal adhesions (FM) as markers of adhesion. The length of FM visualized by immunofluorescent labelling of talin 1 in human keratinocytes (HaCaT) cultured on selected Ar plasma modified matrices was greater at 24 h than at 72 h. The number of FM per cell with FM increased for some of the modified surfaces during the 72 h period and the percentage of cells with FM mildly decreased. Furthermore, we analyzed the percentage of live HaCaT cells on the tested matrices by means of live/dead staining, with cells reaching almost 100% viability on modified FEP matrices. Altogether, Ar plasma modification positively affected viability and adhesion of HaCaT cells on FEP. Both these features may be related because formation of focal adhesions not only enables cell adhesion but also affects intracellular signaling. We also show that the assessment of suitable surface modification of biocompatible materials should be more complex than mere evaluation of viability or number of the cells on the surface.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20501 - Materials engineering
Result continuities
Project
<a href="/en/project/GBP108%2F12%2FG108" target="_blank" >GBP108/12/G108: Preparation, modification and characterization of materials by radiation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Materials science and engineering C
ISSN
0928-4931
e-ISSN
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Volume of the periodical
100
Issue of the periodical within the volume
JUL 2019
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
269-275
UT code for WoS article
000466059700026
EID of the result in the Scopus database
2-s2.0-85062466390