Argon plasma-treated fluorinated ethylene propylene: Growth of primary dermal fibroblasts and mesenchymal stem cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F19%3A43918616" target="_blank" >RIV/60461373:22310/19:43918616 - isvavai.cz</a>
Alternative codes found
RIV/60461373:22330/19:43918616
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0040816619300655" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0040816619300655</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.tice.2019.05.004" target="_blank" >10.1016/j.tice.2019.05.004</a>
Alternative languages
Result language
angličtina
Original language name
Argon plasma-treated fluorinated ethylene propylene: Growth of primary dermal fibroblasts and mesenchymal stem cells
Original language description
Surface modification is an important step in making a synthetic polymer cytocompatible. We have previously reported improved cytocompatibility of immortalized human keratinocytes (HaCaT) with the otherwise bioinert fluorinated ethylene propylene (FEP) upon treatment with argon plasma discharge. In this article, we show that FEP modified with Ar plasma with the power of 3 and 8W for 40 and 240 s served as a suitable material for cultivation of primary human dermal fibroblasts (HDF), which showed significantly improved proliferation and spreading comparable to standard tissue culture polystyrene. We also evaluated focal adhesions formed by HDF cells on modified FEP, which were far more numerous compared to pristine FEP. Moreover, we attempted spontaneous osteogenic differentiation of adipose-derived mesenchymal stem cells modified with human telo-merase reverse transcriptase on Ar plasma-modified FEP. While the spontaneous osteogenic differentiation was unsuccessful, the cells were able to adhere and differentiated on tested matrices upon the administration of osteodifferentiation medium. These combined findings suggest that the treatment of FEP with Ar plasma comprises and efficient method to enable the adhesion and proliferation of various cell types on an otherwise largely bioinert material.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Tissue and Cell
ISSN
0040-8166
e-ISSN
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Volume of the periodical
58
Issue of the periodical within the volume
June
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
121-129
UT code for WoS article
000468871600017
EID of the result in the Scopus database
2-s2.0-85065703472