Reduced lipid and glucose oxidation and reduced lipid synthesis in AMPKα2-/- myotubes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F25%3A00636708" target="_blank" >RIV/67985823:_____/25:00636708 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1080/13813455.2024.2449409" target="_blank" >https://doi.org/10.1080/13813455.2024.2449409</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/13813455.2024.2449409" target="_blank" >10.1080/13813455.2024.2449409</a>
Alternative languages
Result language
angličtina
Original language name
Reduced lipid and glucose oxidation and reduced lipid synthesis in AMPKα2-/- myotubes
Original language description
Adenosine 5’-monophosphate (AMP)-activated protein kinase (AMPK) plays a crucial role in regulation of metabolic homeostasis. To understand the role of the catalytic α2 subunit of AMPK in skeletal muscle energy metabolism, myotube cultures were established from AMPKα2+/+ and AMPKα2-/- mice. Myotubes from AMPKα2-/- mice had lower basal oleic acid and glucose oxidation compared to myotubes from AMPKα2+/+ mice. However, the relative response to mitochondrial uncoupling was increased for oleic acid oxidation. Incorporation of acetate into lipids was also lower in myotubes from AMPKα2-/- mice. Proteomics analysis revealed that AMPKα2-/- myotubes had upregulated pathways related to mitochondrial function and fatty acid oxidation, and decreased pathways related to fatty acid biosynthesis. In conclusion, ablation of AMPKα2 catalytic subunit in skeletal muscle cells resulted in reduced basal oxidation of glucose and fatty acids, however upregulated pathways related to mitochondrial function and fatty acid oxidation and reduced lipid formation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Archives of Physiology and Biochemistry
ISSN
1381-3455
e-ISSN
1744-4160
Volume of the periodical
131
Issue of the periodical within the volume
3
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
483-492
UT code for WoS article
001392784900001
EID of the result in the Scopus database
2-s2.0-85214662658