3D Microstructural Characterization of Human Deep Fascia Using Optical Projection Tomography, Digital Light Sheet Microscopy, and Magnetic Resonance Microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F25%3A00640547" target="_blank" >RIV/67985823:_____/25:00640547 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1002/jemt.70035" target="_blank" >https://doi.org/10.1002/jemt.70035</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jemt.70035" target="_blank" >10.1002/jemt.70035</a>

Result language

angličtina

Original language name

3D Microstructural Characterization of Human Deep Fascia Using Optical Projection Tomography, Digital Light Sheet Microscopy, and Magnetic Resonance Microscopy

Original language description

Traditional histological methods provide limited insights into the complex 3D microstructure of fascia and its relationship to disease. This study explored the capacity of different 3D microscopy techniques for characterizing the microstructure of fascia lata (FL) and thoracolumbar fascia (TLF). Tissues from four donors were studied using optical projection tomography (OPT), digital light sheet (DLS) microscopy, and magnetic resonance microscopy (MRM). Samples for OPT and DLS were imaged with a custom OPT scanner and the DLS arm of a Leica Stellaris microscope, respectively. MRM was performed using a 9.4 T superconducting magnet and an NMR/MRI spectrometer. Reference histological evaluation was performed to guide the interpretation of 3D data. Image analyses were performed using FIJI and Ellipse software. DLS offered superior resolution, but all techniques revealed a trilaminar structure in both fasciae: a thick, collagen-rich intermediate layer flanked by thinner layers with loose connective tissue. The FL intermediate layer was thinner (210.5–258.7 μm) with longitudinally oriented collagen, while the TLF intermediate layer was thicker (302.3–343.6 μm) with both oblique and longitudinal fibers. The superficial layer in FL was thicker (128.8–161.5 μm) than in TLF (84.65–123.10 μm) across imaging modalities. The deep layer also varied between fasciae, with 54.3–73.8 μm in FL and 44.78–70.30 μm in TLF. Layer thickness measurements did not differ significantly across techniques. This study demonstrates the feasibility of different 3D microscopy techniques for visualizing and quantifying fascia extracellular matrix structure and organization, laying the groundwork for future investigations into potential structural alterations in disease.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30106 - Anatomy and morphology (plant science to be 1.6)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microscopy Research and Technique

ISSN

1059-910X

e-ISSN

1097-0029

Volume of the periodical

88

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

3037-3049

UT code for WoS article

001523672700001

EID of the result in the Scopus database

2-s2.0-105009884930