Near-Critical Extraction of ß-Sitosterol and Scopoletin from Stinging Nettle Roots

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F05%3A00028586" target="_blank" >RIV/67985858:_____/05:00028586 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Near-Critical Extraction of ß-Sitosterol and Scopoletin from Stinging Nettle Roots

Original language description

ß-Sitosterol (22,23-dihydrostigmasterol) and scopoletin (7-hydroxy-6-methoxycoumarin) were extracted from roots of stinging nettle (Urtica dioica L.) with supercritical and liquid CO2. Extraction rates were measured for both oleoresin and the individualsubstances in dependence on extraction conditions. The solvent modified with ethanol (0?9.4 wt.%) was applied at the pressures of 100, 200, 250 and 280 bar and the temperatures of 25, 40 and 60 °C. Extracts were analysed by means of HPLC method. The maximum yields of ß-sitosterol and scopoletin were 0.63 and 0.058 mg/g dry mass, respectively. In comparison with the extract obtained with diethyl ether, the maximum yields obtained with CO2 were at least two times higher for both investigated substances. Amodel describing the extraction of a solute that is present in two forms, free and interacting with matrix, was applied to interpret the experimental extraction curves.

Czech name

Extrakce ß-sitosterolu (22,23-dihydrostigmasterol) a skopoletinu (7-hydroxy-6-methoxykumarin) superkritickým a kapalným CO2 z kořenů kopřivy (Urtica dioica L.) a kapalným CO2

Czech description

Z kořenů kopřivy (Urtica dioica L.) byly superkritickým a kapalným CO2 extrahovány ß-sitosterol (22,23-dihydrostigmasterol) a skopoletin (7-hydroxy-6-methoxykumarin). Byla změřena rychlost extrakce jak celkového extraktu, tak i obou jeho složek. Extrakční podmínky byly: tlak 100, 200, 250 a 280 bar, teplota 25, 40 a 60 °C, koncentrace unašeče (ethanolu) 0-9.4 % hm. Extrakty byly analyzovány kapalinovou chromatografií. Maximální výtěžky (0,63 mg/g sušiny u ß-sitosterolu a 0,058 mg/g sušiny u skopoletinu)byly nejméně dvojnásobné oproti výtěžkům extrakce diethyleterem. Změřené extrakční křivky byly interpretovány modelem, který rozlišuje dvě formy extrahované látky v rostlině, volnou látku a látku vázanou na matrici.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CI - Industrial chemistry and chemical engineering

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Supercritical Fluids

ISSN

0896-8446

e-ISSN

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Volume of the periodical

35

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

111-118

UT code for WoS article

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EID of the result in the Scopus database

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