Optical Fiber Elements for Increasing Bioluminescence Coupling

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F09%3A00337995" target="_blank" >RIV/67985858:_____/09:00337995 - isvavai.cz</a>
Alternative codes found
RIV/67985882:_____/09:00337995 RIV/67985858:_____/09:00375644 RIV/67985882:_____/09:00375644
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Optical Fiber Elements for Increasing Bioluminescence Coupling
Original language description
Optical fiber biosensors based on monitoring of cell bioluminescence are employed as environmental pollution sensors. An active layer containing bioluminescent cells on the fiber element end face is a basal part of an optical fiber biosensor (OFB). The number of immobilized living cells and light coupling efficiency determine of OFB sensitivity. In this paper, two shapes of optical fiber elements (OFE) are compared; 600 ?m polymer cladded silica (PCS) fiber, and tapered quartz element with 6.3 mm diameter on the front end narrowing to 800 ?m (length 32 cm). On these ends, cells Pseudomonas putida TV A8 were immobilized in silica matrix. Bioluminescence measurements confirmed a proportionality between the number of cells and bioluminescence. The numberof immobilized cells was 105 times higher on the tapered OFE end as compared to the 600 ?m PCS fiber. The measured bioluminescence intensity increased 4 times in the tapered OFE case.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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