A biosensor with encapsuled bioreporters—optical fiber element for enhanced detectection of bioluminescence
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F18%3A00321984" target="_blank" >RIV/68407700:21460/18:00321984 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1088/1361-6501/aac633" target="_blank" >http://dx.doi.org/10.1088/1361-6501/aac633</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1088/1361-6501/aac633" target="_blank" >10.1088/1361-6501/aac633</a>
Alternative languages
Result language
angličtina
Original language name
A biosensor with encapsuled bioreporters—optical fiber element for enhanced detectection of bioluminescence
Original language description
It is proposed that a whole-cell optical fiber biosensor is used as an in situ first warning detector sensor for online monitoring of environmental pollution (e.g. waste water) in remote localities. The base requirement for this sensor is a very high sensitivity topollution. The work shows a simple way to increase sensor sensitivity, optimizing the shape of an optical fiber element (OFE) for more effective coupling of the low intensity light produced by bioluminescent bioreporters. Theoretical calculations of coupling efficiency showed that only 3.5% of photons produced by one bacterium placed on the front end of polymer-clad silica (PCS) fiber is guided to the detector. Our designed tapered OFEs with immobilized bioluminescent bioreporters Pseudomonas fluorescens HK44, with maximal transmittance, led to a sixfold increase in detected bioluminescence compared to that of the PCS fiber with the same active layer. It is shown that the shape of the OFE determines its transmission. The influences of physical properties and OFE geometry, cell immobilization into silica gel or bacterial plaque, on the intensity of detected light, were evaluated.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20201 - Electrical and electronic engineering
Result continuities
Project
<a href="/en/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: BIO-XUV Research Team Advancement at FBME CTU</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Measurement Science and Technology
ISSN
0957-0233
e-ISSN
1361-6501
Volume of the periodical
29
Issue of the periodical within the volume
7
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
1-7
UT code for WoS article
000435378000001
EID of the result in the Scopus database
2-s2.0-85048984380