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A biosensor with encapsuled bioreporters—optical fiber element for enhanced detectection of bioluminescence

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F18%3A00321984" target="_blank" >RIV/68407700:21460/18:00321984 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1088/1361-6501/aac633" target="_blank" >http://dx.doi.org/10.1088/1361-6501/aac633</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1088/1361-6501/aac633" target="_blank" >10.1088/1361-6501/aac633</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    A biosensor with encapsuled bioreporters—optical fiber element for enhanced detectection of bioluminescence

  • Original language description

    It is proposed that a whole-cell optical fiber biosensor is used as an in situ first warning detector sensor for online monitoring of environmental pollution (e.g. waste water) in remote localities. The base requirement for this sensor is a very high sensitivity topollution. The work shows a simple way to increase sensor sensitivity, optimizing the shape of an optical fiber element (OFE) for more effective coupling of the low intensity light produced by bioluminescent bioreporters. Theoretical calculations of coupling efficiency showed that only 3.5% of photons produced by one bacterium placed on the front end of polymer-clad silica (PCS) fiber is guided to the detector. Our designed tapered OFEs with immobilized bioluminescent bioreporters Pseudomonas fluorescens HK44, with maximal transmittance, led to a sixfold increase in detected bioluminescence compared to that of the PCS fiber with the same active layer. It is shown that the shape of the OFE determines its transmission. The influences of physical properties and OFE geometry, cell immobilization into silica gel or bacterial plaque, on the intensity of detected light, were evaluated.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    20201 - Electrical and electronic engineering

Result continuities

  • Project

    <a href="/en/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: BIO-XUV Research Team Advancement at FBME CTU</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Measurement Science and Technology

  • ISSN

    0957-0233

  • e-ISSN

    1361-6501

  • Volume of the periodical

    29

  • Issue of the periodical within the volume

    7

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    7

  • Pages from-to

    1-7

  • UT code for WoS article

    000435378000001

  • EID of the result in the Scopus database

    2-s2.0-85048984380