A biosensor with encapsuled bioreporters—optical fiber element for enhanced detectection of bioluminescence

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F18%3A00321984" target="_blank" >RIV/68407700:21460/18:00321984 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1088/1361-6501/aac633" target="_blank" >http://dx.doi.org/10.1088/1361-6501/aac633</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1088/1361-6501/aac633" target="_blank" >10.1088/1361-6501/aac633</a>

Result language
angličtina
Original language name
A biosensor with encapsuled bioreporters—optical fiber element for enhanced detectection of bioluminescence
Original language description
It is proposed that a whole-cell optical fiber biosensor is used as an in situ first warning detector sensor for online monitoring of environmental pollution (e.g. waste water) in remote localities. The base requirement for this sensor is a very high sensitivity topollution. The work shows a simple way to increase sensor sensitivity, optimizing the shape of an optical fiber element (OFE) for more effective coupling of the low intensity light produced by bioluminescent bioreporters. Theoretical calculations of coupling efficiency showed that only 3.5% of photons produced by one bacterium placed on the front end of polymer-clad silica (PCS) fiber is guided to the detector. Our designed tapered OFEs with immobilized bioluminescent bioreporters Pseudomonas fluorescens HK44, with maximal transmittance, led to a sixfold increase in detected bioluminescence compared to that of the PCS fiber with the same active layer. It is shown that the shape of the OFE determines its transmission. The influences of physical properties and OFE geometry, cell immobilization into silica gel or bacterial plaque, on the intensity of detected light, were evaluated.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20201 - Electrical and electronic engineering

Project
<a href="/en/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: BIO-XUV Research Team Advancement at FBME CTU</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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