Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F12%3A00202769" target="_blank" >RIV/68407700:21460/12:00202769 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Whole-cell biosensor- Influence of optical fiber element shape on detection of low-level bioluminescence
Original language description
The whole-cell biosensor which used a special optical fiber element (OFE) from pure silica with an active layer contents bioluminescent bioreporters was developed as a real in-situ detector. The sensitivity depends on a value of detected bioluminescence(BL), which can be very low. In this study, we explore the influence of OFE shape on BL transition as well as number of cells immobilized into the active layer on detecting low-level bioluminescent signals. The bioluminescence signal was produced by thegenetically engineered bacterial bioreporter (light source). Mathematical model of OFE shape was developed based on geometric optics. Four different shapes of OFE were studied experimentally. For development of mathematical model of the optimal OFE shapefrom point of view maximum detected intensity of BLwas applied parameters of the best OFE from experimental measurements. The correspondence between results of calculated transmission by the model and experimental measurements of BL was
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
JB - Sensors, detecting elements, measurement and regulation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: BIO-XUV Research Team Advancement at FBME CTU</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Instuments and Methods for Biology and Medicine 2012
ISBN
978-80-01-05119-1
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
49-51
Publisher name
Czech Technical University
Place of publication
Prague
Event location
Kladno
Event date
May 29, 2012
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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