Mathematical Model for Laboratory System of Bioluminescent Whole-Cell Biosensor with Optical Element.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985858%3A_____%2F18%3A00574904" target="_blank" >RIV/67985858:_____/18:00574904 - isvavai.cz</a>

Result on the web

<a href="https://hdl.handle.net/11104/0344829" target="_blank" >https://hdl.handle.net/11104/0344829</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4172/2155-6210.1000250" target="_blank" >10.4172/2155-6210.1000250</a>

Result language

angličtina

Original language name

Mathematical Model for Laboratory System of Bioluminescent Whole-Cell Biosensor with Optical Element.

Original language description

The fiber-optic biosensor with encapsuled bioreporters use a special optical element (OE) from pure silica with an active layer contents bioluminescent bioreporters was developed as a real in-situ detector for on-line measurement in remote localities. The active layer of biosensor contents bioluminescent bioreporters – genetic modified cells, which are sensitive to its surrounding environment-immobilized in silica matrix on the end of OE. Bioreporters are able to react by emission of visible light (≈500 nm) – bioluminiscence reaction (BL) – in presence of specific analyte.The genetic modified bacterial stain Pseudomonas putida TVA8 was chosen as biorecognition part of biosensor producing BL in the presence of benzene, toluene, ethylbenzene and xylens (BTEX). Very low level of BL signal was detected by high-sensitive photon-counter. The sensitivity of biosensor depends on a value of detected BL and it can be very low. The signal is also affected by number of cells (light sources) immobilized into the active layer nand transmission through OE. Mathematical model of OE shape was developed based on geometric optics. The numerical model is built around the MATLAB scripting language. Simulation of ray transmission was calculated for OE with different shape.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

20902 - Bioprocessing technologies (industrial processes relying on biological agents to drive the process) biocatalysis, fermentation

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biosensors and Bioelectronics

ISSN

2155-6210

e-ISSN

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Volume of the periodical

9

Issue of the periodical within the volume

1

Country of publishing house

BE - BELGIUM

Number of pages

5

Pages from-to

1000250

UT code for WoS article

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EID of the result in the Scopus database

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